摘要
本研究旨在检测红眼白水貂促卵泡激素β(follicle-stimulating hormone beta subunit,FSHβ)和核受体辅激活蛋白1(nuclear receptor coactivator 1,NCOA1)基因多态性与繁殖性状的关系。采用单链构象多态性(SSCP)和DNA测序相结合的方法检测了红眼白水貂215个个体的单核苷酸多态性,针对红眼白水貂群体的特点建立合适的统计分析模型,利用SAS 9.4统计软件对候选基因进行多态性分析,并采用最小二乘法分析了总产仔数和产活仔数的遗传效应。结果表明,在红眼白水貂FSHβ基因上存在2个多态位点,分别为内含子1处的g.1228G>A突变和外显子2处的g.1866T>C突变;在NCOA1基因上存在1个多态位点,为第6外显子处g.151536T>C突变。在红眼白水貂群体中,FSHβ基因的优势基因为B等位基因,NCOA1基因的优势基因为A等位基因;FSHβ基因g.1228G>A位点的AA和AB基因型个体在总产仔数、产活仔数上均极显著高于BB基因型(P<0.01),g.1866T>C位点在总产仔数和产活仔数上呈现BB>AB>AA的趋势;NCOA1基因的AB基因型个体的总产仔数和产活仔数均极显著高于AA基因型(P<0.01);g.151536T>C与g.1228G>A的合并基因型对繁殖性状有显著影响(P<0.05)。因此,可以利用以上突变位点对红眼白水貂的繁殖性状进行标记辅助选择研究。
This study was performed to investigate the effects of follicle-stimulating hormone beta subunit（FSHβ）and nuclear receptor coactivator 1（NCOA1）genes on reproductive traits of pink eye White mink.Single nucleotide polymorphisms（SNPs）were detected in 215 individuals by single-strand conformation polymorphism（SSCP）and DNA sequencing,correlation analysis were carried out between candidate gene polymorphisms and reproductive traits using the appropriatemodel,the effects of different genotypes on reproductive traits,including total number born（TNB）and number born alive（NBA）,were analyzed by the least square analysis of SAS 9.4 software.The results showed that two polymorphic sites were found in FSHβgene,g.1228 GA in intron 1,g.1866 TC in exon 2.One SNP was found in NCOA1 gene,g.151536 TC in exon 6.B allele of FSHβgene was predominant allele,A allele of NCOA1 gene was predominant allele;TNB and NBA of AA and AB genotypes in FSHβg.1228 GA site were extremely significantly higher than BB genotype（P〈0.01）;The TNB and NBA for g.1866C site genotypes were BBABAA;TNB and NBA of NACOA1 gene AB genotype was extremely significantly higher than AA genotype（P〈0.01）;The combing genotypes of 151536 TC-g.1228 GA were significant differences in TNB and NBA（P〈0.05）.Therefore,these SNPs could be used for marker assist selection on reproduction traits of the pink eyed White mink.
出处
《中国畜牧兽医》
CAS
北大核心
2018年第2期429-438,共10页
China Animal Husbandry & Veterinary Medicine
基金
中国农业科学院创新工程(CAAS-ASTIP-2016-ISAPS)
国家自然科学基金(31702152)
