摘要
目的:观察七叶皂苷钠冻干粉对出血性脑水肿大鼠脑组织基质金属蛋白酶-9(MMP-9)及水通道蛋白4(AQP4)的影响。方法:制备大鼠脑出血模型,将实验大鼠随机分为空白组、模型组、七叶皂苷钠组和地塞米松组。七叶皂苷钠组给予七叶皂苷钠冻干粉配置的溶液(3.5mg·kg^(-1)·d^(-1)),地塞米松组给予地塞米松磷酸钠注射液(5.0mg·kg^(-1)·d^(-1)),空白组和模型组给予等体积0.9%氯化钠溶液,腹腔注射,1次/d,连续7d。各组分别于给药后6、24、72、120h断头取脑,每组每个点取8只鼠,收集脑组织标本用作ELISA和RT-q PCR检测。结果:与空白组比较,造模6h后脑组织中MMP-9的表达量开始升高,到72h达到峰值,此后表达量开始降低,到120h时仍未恢复到正常水平(P<0.01)。脑出血大鼠在注射七叶皂苷钠后脑组织中MMP-9的表达量较模型组都有显著降低(P<0.05,P<0.01)。与空白组比较,造模6h后脑组织中AQP4 mRNA的表达量开始升高,到72h达到峰值,此后表达量开始降低,到120h时仍未恢复到正常水平(P<0.01)。给脑出血大鼠应用七叶皂苷钠或地塞米松后,脑组织中AQP4 mRNA的表达量较模型组都有显著降低(P<0.05,P<0.01)。结论:七叶皂苷钠冻干粉能改善大鼠出血性脑组织的水肿程度,其机制可能与抑制脑内MMP-9生成和降低AQP4 mRNA的表达有关。
Objective： To observe the effects of sodium aescinate freeze-dried powder on MMP-9 and AQP4 in brain tissue of hemorrhagic brain edema rats. Methods： The rat model of brain hemorrhagic was prepared. The experimental rats were randomly divided into blank group, model group, sodium aescinate group and dexamethasone group. The sodium aescinate group was given a solution consisted of sodium aescinate freeze-dried powder（3.5 mg · kg^-1 · d^-1）, the dexamethasone group was given dexamethasone sodium phosphate injection（5.0 mg · kg^-1 · d^-1）, and the blank group and model group were given same volume of normal saline, with intraperitoneal injection, once a day for continuous seven days. 6 h, 24 h, 72 h and 120 h after administration, eight rats in each group were sacrificed to collect brain tissue samples for ELISA and RT-q PCR at each time. Results： MMP-9 level detection in brain tissue： compared with the blank group, the expression quantity of MMP-9 in brain tissues began to increase 6 hours after modeling, and it reached its peak valued 72 hours after modeling, then it began to decrease, and it still had not back to the normal level 120 hours after modeling（P〈0.01）. The expression quantity of MMP-9 in intracerebral hemorrhagic rats＇ brain tissues after injection of sodium aescinate was lower than that in the model group（P〈0.05, P〈0.01）. Compared with the blank group, the expression quantity of AQP4 mRNA began to increase 6 hours after modeling, and it reached its peak valued 72 hours after modeling, then it began to decreased, and it still had not back to the normal level 120 hours after modeling（P〈0.01）. The expression quantity of AQP4 mRNA in the brain tissue of intracerebral hemorrhagic rats after sodium aescinate was lower than that in the model group（P〈0.05, P〈0.01）. Conclusion： Sodium aescinate freeze-dried powder can improve the degree of cerebral tissue edema after hemorrhage in rats. The mechanism may be related to inhibiting the expression of MMP-9 in the brain and decreasing the expression of AQP4 mRNA.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第3期1060-1062,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
湖北省科技厅重大科技创新计划项目(No.2013ACC005)
国家重大新药创制科技重大专项(No.2014ZX09201-022)~~
