摘要
菊芋秸秆是一种富含纤维素的生物质资源,为使其充分转化利用,本文探索了菊芋秸秆(JAS)制备食品添加剂微晶纤维素的可行性。以JAS为原料,采用稀盐酸水解法制备微晶纤维素(JASMCC),着重考察盐酸质量分数、水解时间、温度和料液比4个因素对JASMCC产品聚合度和得率等指标的影响,并通过响应面法优化工艺参数。结果表明:菊芋秸秆中纤维素含量高达43.61%以上。经响应面法优化的JASMCC的最佳制备工艺参数为:料液比1∶14 g/m L、盐酸质量分数5%、温度60℃、时间85 min。在此工艺下所得JASMCC产品的聚合度为255.14,得率为36.96%,纯度为97.80%,符合国标GB1886.103-2015《食品添加剂微晶纤维素》要求。利用傅里叶变换红外光谱、扫描电镜和X-射线衍射对JASMCC制备过程中的微观形貌和结晶结构进行表征,并与商业微晶纤维素比较,结果发现JASMCC的微观形态呈棒状,纤维素晶型为Ⅰ型,相对结晶指数为63.42%,说明利用菊芋秸秆制备食品添加剂微晶纤维素的工艺可行。
Jerusalem artichoke, as a renewable resource, is widely distributed around the world. However, the Jerusalem artichoke stalk（JAS）, rich in cellulose, has not been well exploited. This study was aimed at the extraction of MCC from Jerusalem artichoke stalk（JAS） by acid hydrolysis using hydrochloric acid. The effects of liquid-solid ratio,temperature and hydrochloric acid concentration on the yield and degree of polymerization（DP） of MCC from JAS（JASMCC） were analyzed by response surface methodology, which was based on single factor experiments. The JASMCC was characterized by SEM, XRD and FTIR investigations and compared with JAS and commercial MCC（LowaRPH101）.The results showed that the optimum conditions of preparation were as follows： the liquid-solid ratio of 14：1, 5% hydrochloric acid concentration, 60 ℃ and 85 minutes. Under this conditions, the JASMCC with balanced polymerization degree of 255 ± 2, yield of（36.96 ± 0.08）%, cellulose content of（97.80 ± 0.06）%, was obtained. The result of structural characterization showed that the X-ray crystallographic index of JASMCC was 63.42%, far above JAS（47.93%）. FTIR spectroscopy and SEM showed that the main component of JASMCC was cellulose. JASMCC is rod-shaped, and cellulose crystal type is type I. It is feasible to prepare food additive MCC from JAS, which provided a reference for the utilization of JAS.
作者
任海伟
沈佳莉
朱晓倩
张丙云
范文广
王永刚
李志忠
Ren Haiwei;Shen Jiali;Zhu Xiaoqian;Zhang Bingyun;Fan Wenguang;Wang Yonggang;Li Zhizhong(School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 73005)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第1期119-127,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(51666010
51366009)
甘肃省自然科学基金项目(1606RJZA206
1606RJYA287
17JR5RA117)
关键词
菊芋秸秆
微晶纤维素
结构表征
响应面法
Jerusalem artichoke stalk
microcrystalline cellulose
structural characterization
response surface methodology
