摘要
目的:基于网络药理学,探讨"黄芩苷-栀子"的功效物质基础和配伍机制。方法:通过数据库检索获得黄芩苷和栀子的化学成分、作用靶点及相关疾病等信息,构建"药物-成分-靶标-疾病"网络,进行基因本体(gene ontology,GO)分类富集分析、基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的通路富集分析,研究"黄芩苷-栀子"的作用机制。结果:"药物-成分-靶标-疾病"网络包含2个药物,20种活性成分,相应靶标198个,相关疾病369种。关键靶点涉及前列腺素内过氧化物合酶2、过氧化物酶体增殖物激活受体γ和5-脂氧合酶等,关键疾病涉及多种恶性肿瘤等。GO富集分析包含GO条目1 414个,其中生物过程相关条目1 209个,分子功能相关条目143个,细胞组分相关条目62个。KEGG通路富集分析包含KEGG通路20条,主要涉及多种恶性肿瘤信号通路。结论:本研究结果初步验证了"黄芩苷-栀子"的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。
OBJECTIVE： To probe into the material bases of efficacy and mechanism of making prescriptions of＂baicalin-gardenia＂based on the network pharmacology. METHODS： Data of chemical compositions,action targets and related diseases of baicalin and gardenia were retrieved to construct ＂drug-composition-target-disease＂network and conduct classified enrichment analysis of gene ontology（ GO） and pathway enrichment analysis based on Kyoto encyclopedia of genes and genomes（ KEGG）,so as to investigate the functional mechanism of ＂baicalin-gardenia＂.RESULTS： The ＂drug-composition-target-disease ＂ network contained 2 kinds of drugs, 20 kinds of active compositions,198 related targets and 369 kinds of related diseases. Key target spots involved prostaglandinendoperoxide synthase-2,peroxisome proliferator-activated receptor γ and 5-lipoxygenase, key diseases included various malignant tumors and so on. GO enrichment analysis contained 1 414 GO items,of which 1 209 involved biological process,143 involved molecular function and 62 involved cellular compositions. KEGG pathway enrichment analysis contained 20 pathways,mainly were pathways of various malignant tumors. CONCLUSIONS： This study has preliminarily verified the basic pharmacological actions and the mechanism of ＂baicalin-gardenia ＂, and laid foundations for further revealing the functional mechanism.
出处
《中国医院用药评价与分析》
2018年第1期1-6,11,共7页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
国家自然科学基金项目(No.81473547
No.81673829)
关键词
黄芩苷
栀子
网络药理学
靶点
基因本体
信号通路
Baicalin
Gardenia
Network pharmacology
Target spot
Gene ontology
Signaling pathway
