摘要
体外诱导源于成年大鼠的胰腺导管单克隆上皮样干细胞分化形成胰岛、神经、脂肪及成骨细胞,探讨干细胞的多分化潜能。扩增培养源于成年大鼠的胰腺导管单克隆上皮样干细胞,采用不同的诱导液体外诱导其向胰岛、神经、脂肪及成骨细胞分化,并通过DTZ染色、糖刺激试验、免疫荧光反应、油红O染色、茜素红染色或Vonkossa染色的方法对分化细胞进行检测。结果显示,体外诱导培养干细胞分化形成类胰岛,DTZ染色阳性,糖刺激分泌胰岛素、C-肽;分化形成类神经细胞,表达神经元特异性烯醇化酶;分化形成类脂肪细胞,油红O染色阳性;分化形成类成骨细胞,其分泌物呈岛状矿化结节,茜素红和Vonkossa染色阳性。这表明,该源于成年大鼠的胰腺导管上皮样干细胞系具有多分化潜能。
Monoclonal epithelial stem cells derived from pancreatic duct of an adult rat were in vitro induced to differentiate into pancreatic islets, nerve cells, adipose cells and osteoblasts. And pluripotency of these stem cells was studied. Monoclonal epithelia stem cells derived from pancreatic duct of an adult rat were proliferated. With different inducement medium, epithelial stem cells were respectively induced to differentiate into pancreatic islets, nerve cells, adipose cells and osteoblasts. Characteristics of differentiated cells were respectively detected with DTZ staining, glucose stimulation experiment, immune-fluorescence reaction, oil red staining, alizarin red staining or Vonkossa staining. The results showed that in inducement culture conditions, monoclonal epithelial stem cells derived from pancreatic duct of an adult rat differentiated into functional pancreatic islet-like tissue that was positive for DTZ staining, secreted insulin and C-peptide using glucose stimulation. Monoclonal epithelial stem cells were induced to differentiate into nerve-like cells which expressed neuron specific enolase, and were induced to differentiated into adipose-like cells that displayed accumulation of lipid vacuoles as detected by oil red O. Also stem cells were induced to differentiate into osteoblast-like cells that excretion formed mineralized nodules. Alizarin red or Vonkossa staining was positive. This indicated that the monoclonal epithelial stem cells derived from pancreatic duct of an adult rat were pluripotent stem cells that could differentiate into cells from three germ layers.
出处
《中国细胞生物学学报》
CAS
CSCD
2018年第1期41-46,共6页
Chinese Journal of Cell Biology
基金
广东省自然科学基金(批准号:10152408801000023)
教育部留学基金(批准号:教外司留[2010]1561号)
广东省创新强校项目(批准号:粤教高函[2014]97号
[2017]54号)
国家大学生创新创业训练计划(批准号:20131056013)
广东省大学生创新创业训练计划(批准号:pdjh2015a0253)资助的课题~~
关键词
胰腺导管干细胞
分化
胰岛
神经细胞
脂肪细胞
成骨细胞
大鼠
pancreatic duct stem cell
differentiation
pancreatic islet
nerve cell
adipose cell
osteoblast
rat
