摘要
目的:探讨氧化苦参碱对三阴性乳腺癌细胞MDA-MB-231生长增殖及其对迁移能力的影响和机制。方法:CCK-8活细胞计数法分析氧化苦参碱不同浓度(0、1、2、4、6、8、10、20 mg/m L)在不同时间(24、48、72 h)对三阴性乳腺癌细胞MDA-MB-231生长增殖的影响,流式细胞仪检测细胞周期和凋亡,Transwell法观察氧化苦参碱对MDA-MB-231细胞迁移和侵袭能力的影响,QPCR法检测EMT相关因子Snail-2和E-cadherin表达水平。结果:与对照组比较,浓度为2、4、6、8、10、20 mg/m L的氧化苦参碱处理MDA-MB-231细胞24、48和72 h后,对细胞的生长呈时间和剂量依赖性的抑制作用。流式细胞实验提示2 mg/m L氧化苦参碱可使G0/G1期细胞数明显增加(P<0.05),S期和G2/M期的细胞数明显减少(P<0.05),细胞生长阻滞于G0/G1期。中、高浓度氧化苦参碱(4 mg/m L、6 mg/m L)处理12 h能明显抑制MDA-MB-231的侵袭能力,抑制Snail-2 mRNA的表达(P<0.01),上调E-cadherin mRNA和蛋白水平的表达(P<0.01)。结论:氧化苦参碱对三阴性乳腺癌细胞生长和迁移侵袭有抑制作用,具有潜在的抗肿瘤生长和转移作用。
AIM： To study the inhibitory effects of oxymatrine on growth and migration of human triple-negative breast cancer MDA-MB-231 cell.METHODS： CCK-8 assay was used to detect the inhibitory effects of oxymatrine on the growth of MDA-MB-231 cells. Inhibitory effect of oxymatrine on cell cycle of MDA-MB-231 cells was detected by flow cytometry（ FCM）. Effect of oxymatrine on migration of MDA-MB-231 cells was detected by scratch experimental. RESULTS： Compared with the control group,the growth of MDA-MB-231 cells decreased significantly in a time-and dose-dependent manner after oxymatrine treatment（ 2,4,6,8,10,20 mg/m L for 24,48 and 72 h）. Oxymatrine significantly increased the number of cells in G0/G1 phase and decreased the number of cells in S and G2/M phase（ P〈0. 05）. Transwell showed that oxymatrine treated MDA-MB-231 cells for 12 h significantly inhibited the invasion of MDA-MB-231.The high concentration of oxymatrine significantly up-regulated the expression of E-cadherin and inhibited the expression of Snail-2 mRNA（ P〈0. 01）.CONCLUSION： Oxymatrine exerts its antitumor effect by inhibiting the growth and invasion of MDAMB-231 cells,which plays a potential role on antitumor growth and metastasis.
作者
蔡加琴
魏晓霞
黄旭慧
庄捷
孙红
CAI Jiaqin;WEI Xiaoxia;HUANG Xuhui;ZHUANG Jie;SUN Hong(Department of Pharmacy, Fujian Provincial Hospital, Fuzhoa 350001, Fujian, China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2018年第1期13-17,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金(81403021)
福建省卫生计生青年科研课题(2016-1-7)
福建医科大学启航基金项目计划(2016QH107)
