摘要
目的:探讨双PI3K/m TOR抑制剂NVPBEZ235对宫颈癌Hela细胞的放射增敏作用。方法:四甲基偶氮唑盐(MTT)及集落克隆法测定各因素对Hela细胞的抑制作用。通过流式细胞仪检测药物联合射线(irradiation,IR)对宫颈癌细胞周期的影响。结果:与对照组相比,NVPBEZ235在体外能够抑制人宫颈癌细胞的生长。NVPBEZ235、IR联合作用细胞,在体外可以使细胞周期阻滞于G_2/M期,二者具有协同作用,联合作用效果显著大于单药作用。结论:20%IC50NVPBEZ235在体外能够促进细胞对射线照射的敏感性,抑制细胞的增殖从而实现对宫颈癌细胞的放射增敏作用。
AIM： To investigate the effect that external irradiation of dual PI3 K/m TOR inhibitors NVPBEZ235 on cervical cancer Hela cells. METHODS： Cervical cancer Hela cells treated with different concentrations of NVPBEZ235 and the radiotherapy（ irradiation,IR） were examined for cell viability using MTT assay and colony forming ability. The cycle arrest of cervical cancer cells combined drug with IR was analyzed by using the flow cytometry.RESULTS： Compared with the control group,NVPBEZ235 can inhibit human cervical carcinoma cell in vitro（ P〈0. 05）. The combined use of NVPBEZ235 significantly enhanced the inhibitory effect of IR（ P〈0. 05） in Hela cells. In vitro,NVPBEZ235 and IR,which had synergistic effect,can block cell cycle to G_2/M period by the combined effect which was significantly greater than the effect of one single drug（ P〈0. 05）. CONCLUSION：20% IC50 NVPBEZ235 can promote the cell＇s radiation sensitivity,inhibit the proliferation of cells,and thereby, increase its radio sensitivity to cervical cancer cells.
作者
郑迎奥
李胜泽
张雷
郭祥瑞
鲍佳茜
吴涛
刘健
ZHENG Yingao;LI Shengze;ZHANG Lei;GUO Xiangrui;BAO Jiaqian;WU Tao;LIU Jian(Department of Oncology , the First Affiliated Hospital of Bengbu Medical College;Department of Oncology and Radiotherapy, 3Department of Obstetrics and Gynecology, Bengbu 233004, Anhui , China;Bengbu Medical College Biological Science Department, Bengbu 233000, Anhui, China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2018年第1期18-23,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省教育厅自然科学基金(KJ2015B041
KJ2015B096)
大学生创业创新项目(19262
201610367002)
