摘要
为了阐述玉米叶夹角的遗传基础并定位相关的数量性状位点,利用289份常用玉米自交系为试验材料,在自然条件下测量玉米穗上叶夹角,对其进行性状分析,结果表明,各个环境的穗上叶夹角数据呈正态分布,并且各个环境间的数据呈极显著正相关。同时采用MaizeSNP50基因芯片进行基因分型,利用R平台下的farmCPU对叶夹角进行全基因组关联分析。在4个环境中共检测到42个与玉米叶夹角显著关联(P<0.000 01)的SNP,其中1,5,8号染色体在4个环境下都检测到与穗上叶夹角紧密关联的SNP标记。对筛选出的所有标记综合分析,共鉴定出15个与玉米叶夹角相关的SNP标记位点,分布在Bin1.02、Bin1.03、Bin1.06、Bin1.11、Bin2.05、Bin3.04、Bin5.03、Bin5.04、Bin8.03、Bin8.04、Bin10.07处。采用全基因组关联分析的方法发掘叶夹角基因位点及候选基因,对揭示玉米叶夹角的遗传机理,加速玉米育种进程具有重要的意义。
To illustrate the genetic basis of maize leaf angle and locate the relevant quantitative trait loci,using 289 maize inbred lines was commonly used as test materials,under natural conditions on the measurement of corn leaf angle,analyzing its traits,the results showed that each environment on the ear of leaf angle data normally distributed,and the data had very significantly positive correlation between the various environments. At the same time,the genotyping of the MaizeSNP50 gene chip was carried out,and the whole genome correlation analysis was carried out using farmCPU. Detected in four environments the communist party of China and corn leaf angle significant correlation（ P 0. 000 01） of SNP,chromosome 1,5,8 of them in four conditions were detected and ear leaf angle was closely related to SNP marker. Comprehensive analysis to filter out all the tags that were identified in 15 SNP marker loci associated with maize leaf angle distribution in Bin1. 02,Bin1. 03,Bin1. 06,Bin1. 11,Bin2. 05,Bin3. 04,Bin5. 03,Bin5. 04,Bin8. 03,Bin8. 04,Bin10. 07 place. It is important to explore the genetic mechanism of maize leaf angle and accelerate maize breeding process by using the method of genome-wide association analysis to discover the gene locus of leaf and the candidate genes.
出处
《华北农学报》
CSCD
北大核心
2018年第1期60-64,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31371636)
山东省现代农业产业技术体系玉米产业创新团队项目(SDAIT-01-022-01)
山东省农业良种工程
山东省农业生物资源创新利用研究课题
青岛市应用基础研究计划项目(14-2-4-13-jch)
关键词
叶夹角
相关性分析
关联分析
Length angle
Correlation analysis
Association analysis
