摘要
目的探讨落新妇苷对抑郁症小鼠的抗抑郁作用,并初步探讨其作用机制。方法将72只C57BL/6J雄性小鼠随机分为对照组、模型组、落新妇苷(AST)低剂量组、落新妇苷中剂量组、落新妇苷高剂量组及丙米嗪(IMI)组,每组12只。对照组小鼠正常饲养,模型组、AST低剂量组、AST中剂量组、AST高剂量组及IMI组小鼠给予为期3周的慢性不可预见性应激,制备抑郁症模型,同时分别每天腹腔注射生理盐水、10、20、40 mg·kg-1AST和10 mg·kg-1IMI,然后进行悬尾、强迫游泳、糖水消耗以及敞箱实验等,对6组小鼠的行为学变化进行检测。采用高效液相色谱法(HPLC)检测各组小鼠前额叶皮层内多巴胺(DA)及5-羟色胺(5-HT)水平。结果对照组、模型组、IMI组及AST低、中、高剂量组小鼠水平运动次数和垂直运动次数两两比较差异均无统计学意义(P>0.05)。与对照组比较,模型组小鼠悬尾实验和强迫游泳实验中持续不动时间显著增加(P<0.05)、糖水偏嗜度及前额叶皮层DA和5-HT水平显著降低(P<0.05);与模型组比较,AST低、中、高剂量组和IMI组小鼠在悬尾实验和强迫游泳实验中持续不动时间均显著降低(P<0.05),糖水偏嗜度及前额叶DA水平显著升高(P<0.05);AST中、高剂量组和IMI组小鼠前额叶皮层5-HT水平高于模型组(P<0.05),ASP低剂量组小鼠前额叶皮层5-HT水平与模型组比较差异无统计学意义(P>0.05)。对照组、IMI组及AST低、中、高剂量组小鼠在悬尾实验和强迫游泳实验中持续不动时间及糖水偏嗜度组间两两比较差异均无统计学意义(P>0.05)。结论落新妇苷对抑郁症小鼠具有显著的抗抑郁作用,其作用机制可能与上调前额叶皮层内DA和5-HT水平有关。
Objective To evaluate the anti-depression effect of astilbin on mice with depressive disorder and to explore its mechanism of action. Methods Seventy-two male mice（ C57 BL/6 J type） were randomly divided into control group,model group,low,middle,high dose of astilbin group and imipramine（ IMI） group,with 12 mice in each group. The mice in the control group were adopted in a normal way. The rats in the model group,low,middle,high dose of astilbin group and IMI group were adopted in chronic unexpected mild stress（ CUMS） to establish depression model,and the stress was continuously kept for three weeks and daily medicine with physiological saline,10,20,40 mg·kg^-1 astilbin and 10 astilbin imipramine were provided in the way of intraperitoneal injection. Examination for behavioural changes of animals was implemented via tail suspension test,forced swimming test,sucrose preference test,open field test. High performance liquid chromatography（ HPLC） was adopted to examine the level of dopamine（ DA） and 5-hydroxytryptamine（ 5-HT） in prefrontal cortex. Results There was no significant difference in the number of horizontal movement and vertical movement among the control group,model group,IMI group and low,middle,high dose of astilbin group（ P〈0. 05）. Compared with the control group,the dead time of tail suspension test and forced swimming test in the model group was longer（ P〈0. 05）,and sugar preference,DA and 5-HT in prefrontal cortex decreased dramatically（ P〈0. 05）. Compared with the model group,the dead time of tail suspension test and forced swimming test in the low,middle,high dose of astilbin group was shorter（ P〈0. 05）,and sugar preference and DA level in prefrontal cortex increased dramatically（ P〈0. 05）. The 5-HT level in prefrontal cortex in the middle,high dose of astilbin group and IMI group was higher than that in the model group（ P〈0. 05）,but there was no significant difference in the 5-HT level in prefrontal cortex between low dose of astilbin group and model group（ P〈0. 05）. There was no significant difference in the dead time of tail suspension test and forced swimming test and sugar preference among the control group,IMI group and low,middle,high dose of astilbin group（ P〈0. 05）. Conclusion Astilbin works excellently in anti-depression,and the major mechanism may involve in up-regulating the level of DA and 5-HT in prefrontal cortex.
出处
《新乡医学院学报》
CAS
2018年第1期12-15,21,共5页
Journal of Xinxiang Medical University
基金
河南省教育厅科学技术研究重点项目(13A310865)
