摘要
目的：观察黄连-吴茱萸组分配伍（小檗碱、吴茱萸碱配伍）对高脂血症模型大鼠肝脏Ob-Rb、JAK2、STAT3 mRNA表达的影响。方法：给予高脂饲料喂养建立高脂血症大鼠模型,筛选造模成功大鼠随机分为空白组,模型组,辛伐他汀组,黄连吴茱萸组分配伍高、中剂量组,分别给予相应药物灌胃治疗,干预8周后,检测各组动物血清TC、TG、HDL-C及LDL-C含量;检测各组动物肝指数及肝脏TC、TG水平;RT-PCR法检测肝脏Ob-Rb、JAK2、STAT3 mRNA表达。结果：黄连吴茱萸组分配伍中、高剂量组能显著降低高脂模型大鼠血清TC,LDL-C含量（P〈0.05或P〈0.01）、改善高脂模型大鼠肝脏脂肪病变,以及降低肝脏TG水平（P〈0.05）。与模型组相比,黄连吴茱萸组分配伍中剂量组肝脏Ob-Rb,JAK2,STAT3 mRNA表达明显下降（P〈0.05）。结论：黄连-吴茱萸组分配伍可能通过下调高脂模型大鼠肝脏Ob-Rb,JAK2和STAT3 mRNA的表达参与血脂调控。
Objective： To illustrate effects of Huanglian-Wuzhuyu component compatibility on expressions of hepatic leptin receptor（ Ob-Rb）,janus activated kinase signaltransducer 2（ JAK2） and signal transducer and activator of transcription 3（ STAT3） genes in hyperlipidemic rats. Method： After establishing the hyperlipidemic model successfully,SD rats were divided into normal group,model group,simvastatin group,Huanglian-Wuzhuyu component compatibility middle dosage group and Huanglian-Wuzhuyu component compatibility high dosage group. The model rats of hyperlipidemia were fed with high fat diet,while normal control rats were fed with fundamental diet. After 8 weeks treatment,serum triglycerides（ TG）,total cholesterol（ TC）,low-density lipoprotein cholesterol（ LDL-C）,high density lipoprotein cholesterol（ HDL-C） and hepatic TC,TG and hepatic index were detected. RT-PCR method was used to detect the expressions of Ob-Rb,JAK2,and STAT3 genes in rats liver tissues. Result： The results showed that two different dosage of Huanglian-Wuzhuyu component compatibility groups significantly decreased TC and LDL-C contents in serum of the hyperlipidemic rats（ P 0. 05）,meanwhile reducing hepatic TG content of hyperlipidemic rats as well（ P 0. 05）,ameliorating steatosis in liver. Compared with the model group,Huanglian-Wuzhuyu component compatibility middle dosage group descended the expressions of Ob-Rb,JAK2 and STAT3 genes in hepatic tissues（ P 0. 05）. Conclusion： Huanglian-Wuzhuyu component compatibility could regulate the expressions of hepatic Ob-Rb,JAK2 and STAT3 genes in hyperlipidemic rats,which might be contributed to its effect of blood lipid levels of hyperlipidemia model rats.
出处
《中华中医药学刊》
CAS
北大核心
2018年第3期605-608,I0019,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81373545,81703844)
国家重点基础研究发展计划(973计划)项目(2013CB532005)
四川省教育厅基金项目(17ZB0147)
关键词
黄连
吴茱萸
组分配伍
高脂血症
作用机制
Huanglian （ Rhizoma Coptidis ）
Wuzhuyu （ Evodia Rutaecarpa ）
component compatibility
hyperlipi-demia
mechanism
