摘要
目的:探讨防风多糖治疗骨质疏松大鼠的实验效果及作用机制。方法:选取60只SPF级雌性SD大鼠,采用随机数字表分为假手术组(等量生理盐水)、模型组(等量生理盐水)、阳性对照组[乙烯雌酚0.05 mg/(kg·d)]、防风多糖低剂量组[250 mg/kg浓度防风多糖供试液,20 m L/(kg·d)、防风多糖高剂量组500 mg/kg浓度防风多糖供试液,20 m L/(kg·d)]各12只。结果:干预70 d后,模型组股骨干重较假手术组显著降低(P〈0.05),阳性对照组、低剂量组、高剂量组的股骨干重大于模型组(P〈0.05),高剂量组的股骨干重大于低剂量组(P〈0.05);阳性对照组、低剂量组、高剂量组的血清IL-6、TNF-α水平低于模型组(P〈0.05),TGF-β1、NO、NOS高于模型组(P〈0.05);高剂量组的血清IL-6、TNF-α水平低于低剂量组(P〈0.05),TGF-β1、NO、NOS高于低剂量组(P〈0.05);阳性对照组、低剂量组、高剂量组的血清Ca^(2+)、Mg^(2+)、ALP水平低于模型组(P〈0.05),P^(2-)高于模型组(P〈0.05);高剂量组的血清Ca^(2+)、Mg^(2+)、ALP低于低剂量组(P〈0.05),P^(2-)高于低剂量组(P〈0.05)。结论:防风多糖治疗骨质疏松大鼠能有效降低血清中钙、镁离子及ALP浓度,调节细胞因子,缓解去势大鼠的骨高分解状态,达到治疗效果。
Objective: To study the effect and mechanism of the treatment of rats with osteoporosis. Methods: A total of60 SPF grade female SD rats were randomly divided into sham operation group( saline),model group( saline),positive control group[DES 0. 05 mg/( kg·d) ],ledebouriella polysaccharide low dose group[250 mg/kg concentration of Fangfeng polysaccharide test solution,20 m L/( kg·d) ],wind polysaccharide high dose group [500 mg/kg concentration of Fangfeng polysaccharide test solution,20 m L( kg·d) ],12 in each. Results: After 70 days,the model group's femoral shaft weight was significantly decreasedthan those in the sham operation group( P 0. 05). The positive control group,low dose group and high dose group's femur dry weight was greater than that of the model group( P 0. 05). The high dose group's femur dry weight was greater than the low dose group( P 0. 05). The positive control group,low dose group and high dose group's serum IL-6 and TNF-α levels were lower than those of the model group( P 0. 05).TGF-β1,NO and NOS beta 1 were higher than those in the model group( P 0. 05). The high dose group's serum IL-6 and TNF-α levels were lower than those of the low dose group( P 0. 05). TGF-β1,NO and NOS beta 1 were higher than those of the low dose group( P 0. 05). The positive control group,low dose group and high dose group's serum Ca^(2+),Mg^(2+) and ALP were lower than those of the model group( P 0. 05). P^(2-) was higher than that of the model group( P 0. 05). The serum Ca^(2+),Mg^(2+) and ALP of high dose group was than lower than those of the model group( P 0. 05) and P^(2-) was higher than that of the low dose group( P 0. 05). Conclusion: Treatment of osteoporosis in rats with osteoporosis can effectively reduce the concentration of serum calcium,magnesium ions and ALP,regulate cyto-kines,relieve the bone of rats with osteoporosis,and achieve the therapeutic effect.
出处
《中华中医药学刊》
CAS
北大核心
2018年第3期678-680,I0034,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划项目(2014ZQ016)