摘要
目的分析河南省4家大型屠宰场中预冷后肉鸡胴体沙门菌污染水平及耐药状况。方法以河南省4家屠宰量为15 000～50 000羽/日的大型屠宰场为采样点，共采集269份预冷后肉鸡胴体进行沙门菌污染检测，对样品中沙门菌进行检测和分离，采用微量半同体氯化镁孔雀绿最可能数（MPN）法对样品中沙门菌进行定量检验，采用微量肉汤稀释法测定分离株对8种抗生素的最低抑菌浓度（MIC），并进行药敏试验。结果共分离出沙门菌131株，污染率为48.7%，平均污染水平为1.32 MPN/g。131株沙门菌共检出8种血清型，其中肠炎沙门菌最多，为93株（71.0%），其次为印第安纳沙门菌21株（16.0%）。只有2株肠炎沙门菌（1.5%）对所有测试的抗生素敏感，其余129菌株对8种抗生素均有不同程度的耐药，其中对奈啶酸（NAL）的耐药率最高，为79.4%（104株），多重耐药菌株为51株（38.9%）。结论河南省4家大型屠宰场中预冷后肉鸡胴体沙门菌污染率较高，污染水平较为严重，血清型复杂。
Objective Tests were carried out for obtaining contamination level and antimicrobial resistance of Salmonella on broiler carcasses after chilling in four poultry slaughterhouses in Henan.Methods Totally, two hundred sixty nine broiler carcasses after chilling were collected in four slaughterhouses with the daily slaughter amount around 15 000 to 50 000. For qualitative analysis of Salmonella EFSA method was used and for quantitative analysis of Salmonella modified Rappaport-Vassiliadis most probable number （MSRV-MPN） method was used. All of the isolates were subjected to antimicrobial susceptibility testing of 8 antibiotics by minimum inhibitory concentration （MIC）.Results Overall, 48.7% （131/269） of the broiler carcasses after chilling were contaminated by Salmonella, and the average of contamination level is 1.32 most probable number MPN/g. Eight serotypes were detected. The dominant serotype is Salmonella enteritidis （93, 71.0%） followed by Salmonella Indiana （21, 16.0%）. Only 2 （1.5%） Salmonella enteritidis strains were sensitive to all the tested antibiotics and the remaining 129 isolates were resistant to at least one kind of eight class antibiotics. Among them, resistant to NAL was the common （104, 79.4%） and 51 （38.9%） Salmonella isolates were multidrug-resistant.Conclusion The contamination rate and multiple antimicrobial resistant of Salmonella on broiler carcasses after chilling from slaughterhouses was very serious, while the isolates contained various serotypes.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2018年第2期124-128,共5页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金（81402685）
北京市优秀人才培养资助（2015000021223ZK35）
国家科技支撑计划（2012BAK17B10）
关键词
沙门菌感染
抗药性
耐药分析
屠宰场
Salmonella infections
Drug resistance
Antimicrobial resistance analysis
Slaughterhouses
