摘要
南极磷虾生活在南冰洋的南极洲水域,是壳聚糖的新型来源,其具有无污染的天然优势,巨大的生物质存量有利于提高提取的天然生物材料质量稳定性;同时,极端的自然环境可使其具有特殊的理化性质。该研究以南极磷虾虾壳作为原料,通过化学方法提取壳聚糖,并对提取工艺的不同脱钙、脱蛋白以及脱乙酰条件进行探索和优化。结果显示,提取的南极磷虾壳聚糖粉末呈亮白色,脱乙酰度达90.6%,分子量为123 k Da,灰分含量为0.095%。在凝血实验评价中,南极磷虾壳聚糖的止血效果明显优于其他商品化来源的壳聚糖,在止血方面具有潜在应用前景。
Antarctic krill as the dominant prey in the Southern Ocean has astonishing standing biomass. As a new source of chitosan, the unpolluted environment and the huge biomass stock would improve the quality of chitosan for biomedical application. In this study, Antarctic krill shrimp shells were used to extract chitosan by chemical method. The reacting condition in decalcification, deproteinization and deacetylation was optimized. The prepared chitosan extracted from Antarctic krill showed bright white powder, and the degree of deacetylationreached to 90.6%, with the molecular weight around 123 k Da and ash content around 0.095%. In hemostasis evaluation, Antarctic krill chitosan showed significantly superior hemostatic effect to other sources of commercialized chitosan, indicating its great prospects for hemostatic application.
出处
《集成技术》
2018年第1期43-50,共8页
Journal of Integration Technology
基金
广东省科技项目(2016A020222007)
深圳市基础研究项目(JCYJ 20160531174634936)
深圳市孔雀创新团队项目(110811003586331)
关键词
南极磷虾
壳聚糖
止血
Antarctic krill
chitosan
hemostasis
