摘要
本研究利用81对SSR引物和6对SRAP引物组合对23个中山杉品种及其父母本共26个基因型进行了遗传分析及指纹图谱构建。研究表明,至少利用7个SSR位点组合可有效区分22个基因型,所有81个SSR标记均不能准确区分中山杉149与302以及中山杉406与407,在此基础上,利用筛选出的2对SRAP标记可将未区分的4个无性系加以区分,最终构建了26个基因型的DNA指纹图谱,各基因型间遗传相似系数在0.40~0.96之间,通过聚类分析可将26个基因型分为两大类。研究结果为中山杉品种区分、鉴定及分子育种提供了重要的工具。
Genetic relationship and DNA-fingerprinting of 26 individuals of Taxodium （23 hybrids, 3 Male and Female Parents） were investigated using 81 SSR markers and 6 SRAP markers. The results showed that 22 clones could be effectively distinguished by 7 SSR loci combination, but the 4 clones remained could be only subdivided into two groups （T. ‘Zhongshanshan 149＇ and T. ‘zhongshanshan 302＇, T. ‘Zhongshanshan 406＇ and T. ‘Zhongshanshan 407＇） with 81 SSR. However, the 4 clones could be completely differentiated by two pairs of SRAP marker combination. Eventually, the DNA fingerprinting of all 26 clones was constructed with 7 SSR and 2 SRAP. The genetic similarities among individuals range from 0.40-0.96, on which, a phenetic tree showing the 26 clones were divided two clusters. The present results provided the valuable tools for the identification and molecular breeding of T. ‘Zhongshanshan＇.
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第4期1222-1228,共7页
Molecular Plant Breeding
基金
江苏省创新能力建设计划项目(BM2015019)
江苏省农业科技自主创新资金项目[CX(16)1005]共同资助
关键词
中山杉
SSR标记
SRAP标记
指纹图谱
Taxodium ‘Zhongshanshan＇, SSR markers, SRAP markers, Fingerprints
