摘要
[目的]探讨黄芪水提液对于老年大鼠膀胱功能的改善作用以及对膀胱组织学的影响。[方法]取60只雄性SD大鼠,其中3月龄10只及15月龄50只。将3月龄大鼠设为青年组,15月龄大鼠随机分为老年组、维生素E-C混悬液组、黄芪水提液低剂量组、黄芪水提液中剂量组和黄芪水提液高剂量组,每组10只。青年组和老年组以0.9%氯化钠溶液20m L/(kg·d)灌胃;维生素E-C混悬液组以维生素E-C混悬液5mg/(kg·d)灌胃;黄芪水提液低、中、高剂量组分别以黄芪水提液2g/(kg·d)、4g/(kg·d)、8g/(kg·d)灌胃。各组大鼠均每天灌胃1次,连续6周。6周后测定大鼠尿流动力学变化,同时Masson染色观察膀胱逼尿肌纤维化情况。[结果]与青年组相比,老年组大鼠膀胱总容量(total urine volume,TUV)、残余尿量(residual urine volume,RUV)增加,而膀胱漏尿点压力(bladder leak point pressure,BLPP)、腹压漏尿点压力(abdominal leak point pressure,ALPP)均降低(P<0.05)。与老年组相比,维生素E-C混悬液组和黄芪水提液各剂量组RUV明显降低,TUV明显增加(P<0.05),而BLPP、ALPP显著升高,差异具有统计学意义(P<0.05)。Masson染色提示,与青年组相比,老年组大鼠膀胱逼尿肌纤维化增加(P<0.05);与老年组相比,黄芪水提液中剂量组和高剂量组的膀胱组织胶原纤维占比减少,差异具有统计学意义(P<0.05)。[结论]黄芪水提液能够降低老年大鼠残余尿量,提高膀胱容量,增加漏尿点压力,降低膀胱逼尿肌纤维化程度。
[Objective] To investigate the effect of Astragalus aqueous extract on the improvement of bladder function and effect of histology in aging rats. [Methods] Of the 60 male SD rats, 3 months old were 10 and 15 months old were 50, a total of 3 months old rats were divided into young group, 0.9% sodium chloride solution 20mL/（kg·d） intragastric administration; 15 months old rats were divided into aged group with 10 rats, 0.9% sodium chloride solution 20mid （kg＇d） intragastric administration; Vitamin E-C suspension group with 10 rats, Vitamin E-C suspension 5mg/（kg＇d） intragastric administration; Astragalus aqueous extract low dose group, aqueous extract of Astragalus membranaceus 2g/（kg·d） intragastric administration; Astragalus aqueous extract middle dose group, aqueous extract of Astragalus membranaceus 4g/（kg ·d） intragastric administration; Astragalus aqueous extract high dose group, aqueous extract of Astragalus membranaceus 8g/（kg·d） intragastric administration. Rats in each group were given 1 time daily and intragastric administration of 6 months, rats were determined by urodynamic changes and Masson staining of detrusor fibrosis. [Results] Compared with the young group, the total acpacity of aged rats [total urine volume, bladder residual urine volume（TUV）, residual urine volume, RUV）] increased, and bladder leak point pressure（bladder leak point pressure, BLPP）, abdominal leak point pressure（abdominal leak point pressure, ALPP） were lower（P〈0.05）. Compared with the elderly group, RUV of vitamin E-C suspension group and Astragalus aqueous extract each dose group decreased significantly, while TUV increased significantly（P〈0.05）, while BLPP and ALPP significantly increased, the difference was statistically significant（P〈0.05）. Masson staining showed that compared with the young group, aging rats detrusor fibrosis increased（P〈0.05）; compared with the aged group, than the reduction of the dose group and high dose group of bladder tissue collagen fibers in Astragalus aqueous extract, the difference was statistically significant（P〈0.05）. [Conclusion] Astragalus aqueous extract can reduce the residual urine volume, imnrove bladder canacitv, increase urinary leakage nressure, and reduce the degree of detrusor fibrosis in aged rats.
出处
《浙江中医药大学学报》
CAS
2018年第1期29-34,共6页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药科技计划项目(2015ZA102)~~
关键词
黄芪
水提液
自然衰老大鼠
尿动力
逼尿肌
残余尿量
膀胱容量
剂量
Astragalus membranaceus
aqueous extract
natural aging rats
urinary power
detrusor
remained urine
bladder volume
dosage
