摘要
为探讨前期筛选出的5株对好氧细菌、酵母菌和霉菌具有优良抑菌活性乳酸菌的复合物及其与CaCO_3、酶及尿素不同组合对全株玉米青贮发酵品质及营养成分的影响,进一步筛选优质复合青贮生物制剂,共设置7个不同组合添加物进行全株玉米青贮,并设空白对照组,测定青贮3、10和30 d时其发酵品质和营养成分的变化情况。结果表明,添加5株乳酸菌混合剂有提高全株玉米青贮发酵品质和营养品质的趋势;同时添加CaCO_3和复合乳酸菌能进一步增加青贮饲料的乳酸(LA)和乙酸(AA)产量,尤其是AA产量,防止pH值过分降低,也有提高青贮营养品质的趋势;同时添加尿素和复合乳酸菌能进一步提高青贮的AA和氨态氮的含量,防止pH值过分降低,显著提高粗蛋白(CP)含量,从而提高青贮营养品质;纤维素酶和淀粉酶复合物可以提高发酵产物的水溶性碳水化合物(WSC)含量及LA和AA产量,改善青贮发酵品质;复合CaCO_3、尿素、纤维素酶、淀粉酶和复合乳酸菌的添加对提高全株玉米青贮的发酵品质和营养品质的效果最好;全株玉米青贮发酵品质和营养品质在前3 d变化最快,发酵不同阶段不同添加组合的变化不尽一致。
Lactic acid bacteria （LAB） have the ability to suppress aerobic bacteria, yeast and fungi during forage fermentation. The effects of 5 LAB strains on fermentation quality and nutritive value of corn silage were evaluated at days 3, 10 and 30 after being ensiled. LAB tion, and urea in 7 different treatment combination show that a mixture of the 5 LAB strains increased crop silage. The addition of and acetic acid （AA） levels, strains were combined with CaCO3, an enzyme formula S, th with 1 additional blank control treatment. The results e fermentation quality and nutritive value of whole corn CaCO3 with lactic acid bacterial compounds further increased the lactic acid （LA） especially AA. This fermentation pattern is linked to avoidance of excessively low pH and improvement of the silage nutritional quality. A treatment combination of urea and LAB also increased the AA, NH3-N and crude protein （CP） contents, again indicating prevention of excessively low pH and improved silage nutritional quality. A treatment combination of cellulase and amylase with LAB compound improved the quality of silage by increasing the production of water soluble carbohydrate （WSC）, LA and AA. A treatment combination of CaCO3, urea, cellulose and amylase with LAB gave the best overall results, in terms of fermentation and nutritional quality of corn silage. The values for measures of silage fermentation quality and nutritive value changed rapidly in first 3 days of fermentation, and followed a different time course for the different additives.
出处
《草业学报》
CSCD
北大核心
2018年第3期90-97,共8页
Acta Prataculturae Sinica
基金
农业部公益性行业科研专项(20130305907)
农业部公益性行业科研专项(201503134)
兰州市科技局科技计划项目(农业科技攻关
2012-2-159)
甘肃省农业生物技术研究与应用开发项目(GNSW.2012-25)
甘肃省科技重大专项(143NKDC017)
甘肃省科技重大专项(17ZD2NC020)
甘肃省农牧厅秸秆饲料化利用研究专项([2016]269号)资助
关键词
乳酸菌复合物
CACO3
尿素
酶
青贮品质
lactic acid bacterial compound
CaCO3
urea
enzyme
ensiling quality
