摘要
采用水蒸气蒸馏法提取佛手、香橼、柠檬和椪柑挥发油,用气相色谱-质谱(GC-MS)联用技术分析挥发油中的化学成分,并以清除DPPH·率为指标测定挥发油的抗氧化能力,以生长速率法测定挥发油的抑菌能力.结果表明:从佛手、香橼、柠檬和椪柑挥发油中分别分离出40,44,36和27个色谱峰,主要化合物(相对面积>1%)有11,14,14和7种,分别占总峰面积的93.77%,92.56%,93.58%和94.81%,主要成分均为d-柠檬烯和r-松油烯,但含量差异明显;四者共同成分有11种,佛手挥发油中有13种特有成分在香橼、柠檬、椪柑挥发油中均未检测到;相同浓度的4种挥发油生物活性研究发现,佛手挥发油具有较强的清除DPPH·和抑制黑曲霉能力;并且佛手挥发油对青霉也有一定抑制作用,但弱于香橼和柠檬挥发油.由此可知,佛手挥发油与香橼、柠檬、椪柑挥发油在成分和活性方面都具有一定差异,该研究为佛手挥发油的开发利用及后续研究提供一定的基础.
The volatile oils were extracted from fingered citron,citron,lemon and ponkan by steam distillation,and the chemical constituents were analyzed by the GC-MS technology. The antioxidant and anti-fungus activity of volatile oils were assayed by scavenging DPPH· and growth rate method,respectively. The results showed the volatile oils from fingered citron,citron,lemon and ponkan were identified as 40,44,36,27 peaks,and 11,14,14,7 compounds(area of more than 1%) were identified,which accounted for93. 77%,92. 56%,93. 58%,94. 81% of the total peak area,respectively. The main constituents of volatile oils were d-limonene and r-terpinene,whereas the contents were significant different. The volatile oil of fingered citron had 11 species common components and 13 species special components,compared with the vola-tile oil of citron,lemon and ponkan. The results of biological activity of the volatile oils showed that the volatile oil of fingered citron had excellent DPPH· scavenging activity and stronger inhibitory activity against Aspergillus niger. The anti-fungus effect of the volatile oils from citron and lemon against Penicillium was stronger than that of the volatile oil of fingered citron. It could be seen that chemical components and biological activities of the volatile oils from fingered citron,citron,lemon and ponkan had some differences,and would provide a theoretical basis for the further exploitation and utilization of the volatile oil from fingered citron.
出处
《浙江师范大学学报（自然科学版）》
CAS
2018年第1期84-91,共8页
Journal of Zhejiang Normal University:Natural Sciences
基金
浙江省公益技术研究项目(2014C32111)
关键词
挥发油
化学成分
抗氧化
抑真菌
volatile oils
chemical components
antioxidant
anti-fungus
