摘要
目的探讨皮肤毛母细胞瘤与基底细胞癌的临床病理及免疫组化、鉴别诊断要点。方法对已确诊的6例毛母细胞瘤和20例基底细胞癌的石蜡标本行HE染色及CK7、bcl-2、AR、CD10、Ki-67免疫组化染色,观察形态学和免疫组化表达特点。结果毛母细胞瘤来源于基底样生发细胞,肿瘤位于真皮及皮下组织内,不与表皮相连,境界清楚,由分布均匀的伴有毛囊分化的毛母细胞团构成;免疫组化:5例CK7(-),1例弱(+);3例bcl-2(-),3例弱(+);6例AR(-),6例CD10(-),而瘤周间质(+);Ki-67阳性指数10%~20%。基底细胞癌来源于基底样生发细胞,癌细胞与表皮基底部相连,呈浸润性生长,周边癌细胞呈栅栏状排列,周围间质疏松,癌巢和间质之间形成收缩裂隙;免疫组化14例CK7(+),6例弱(+);17例bcl-2(+),3例弱(+);16例AR(+),4例弱(+);20例为癌细胞CD10(+),癌周间质(-);Ki-67阳性指数20%~30%。结论结合镜下组织学特点及免疫表型,有助于两种肿瘤的鉴别诊断。
Objective To explore the morphological features,immunohistochemical phenotype and differential diagnosis of the trichoblastoma and basal cell carcinoma in skin. Methods A total of 6 cases of trichoblastoma and 20 cases of basal cell carcinoma weres studies by using HE staining and immunohistochemical staining on CK7,Bcl-2,AR,CD10,Ki67. The expression of morphological features and immunohistochemical phenotype were observed in tumor cells.Results Trichoblastoma was from the basal germinal cells. Tumor was located in the dermis and subcutaneous tissues,not connected to the skin,clearly bounded by uniform distribution of trichoblastic cells with follicular differentiation. CK7 was weakly positive in 1 case and negative in 5 cases. bcl-2 was weakly positive in 3 cases and negative in 3 cases. AR was negative in 6 cases. 6 cases of trichoblastoma were positive for CD10 in tumor stroma and negative in tumor cells. The rate of Ki67 was about 10% ~ 20%. Basal cell carcinoma was from the basal germinal cells. Tumor was connected to the skin,grown in an invasive way. The surrounding cancer cells were arranged in a fence. It was loosened in surrounding stroma and formed of lkhrinkage crack in between tumor cells and tumor stroma. CK7 was weakly positive in 6 cases and positive in 14 cases. Bcl-2 was weakly positive in 3 cases and positive in 17 cases. AR was weakly positive in 4 cases and positive in 16 cases. CD10 were positive in tumor cells and negative in tumor stroma. The rate of Ki67 was about 20% ~ 30%.Conclusion The differential diagnosis between two types of tumors is helped by the combination of histological characteristics and immunohistochemical phenotype.
作者
吴雪梅
王小明
郭少峰
颜浩
余梦懿
肖柳
WU Xue-mei, WANG Xiao-ming, GUO Shao-feng, YAN Hao, YU Meng-yi, XIAO Liu(Department of Pathology, People＇ s Hospital of Meishan, Meishan 620010, Chin)
出处
《诊断病理学杂志》
2018年第3期201-204,共4页
Chinese Journal of Diagnostic Pathology
关键词
毛母细胞瘤
基底细胞癌
临床病理特点
免疫组化
Trichoblastoma
Basal cell carcinoma
Morphological features
Immunohistochemical phenotype
