摘要
油菜角果内籽粒着生密度影响每果粒数,对油菜产量有直接或间接影响。本研究以不同遗传背景和地理来源的213份甘蓝型油菜品种(系)构成的自然群体为研究对象,利用芸薹属60 K Illumina Infinium SNP芯片进行群体结构、亲缘关系以及连锁不平衡分析;然后基于最优模型对籽粒着生密度及其相关性状进行全基因组关联(GWAS)分析。通过GWAS分析,共检测到10个SNP位点与籽粒着生密度及其相关性状关联。其中与籽粒着生密度关联的标记有2个,表型贡献率分别为9.49%和11.17%;与角果有效长关联的标记有6个,单个位点可解释9.81%~12.17%的表型变异;与每果粒数相关联的标记有2个,分别解释10.44%和10.87%的表型变异。通过分析关联SNP位点的LD区间的基因信息,筛选出16个与籽粒着生密度及其相关性状有关的候选基因,其中KMD4和UGT76C2基因与细胞分裂素的调控有关;AGL104和ADC2基因参与种子的形成过程;MCCB、NGA2和MATE等基因参与侧生器官的生长发育过程,它们的异常表达会导致一些侧生器官的变异。ADC2和UGT76C2两个候选基因可能对籽粒着生密度和每果粒数一因多效性。
Seed density within per silique will affect seed per silique, which have a direct or indirect effect on rapeseed yield. In this study, a population of 213 Brassica napus L. accessions （or lines） from different genetic backgrounds and geographical origins was subjected to assess population structure, relative kinship and linkage dise- quilibrium using Brassica 60 K Illumina Infinium SNP array. Then the optimal model of each trait was selected for genome-wide association analysis （GWAS） of the seed density within per silique and its related traits. A total of 10 SNPs were detected to be associated with the seed density within per silique and its related traits in 2016. 2 SNP sites associated with seed density within per silique were detected, explaining the phenotypic variation of 9. 94% and 11.17% ,respectively. 6 SNP sites associated with valid silique length were detected,explaining the phenotypic varia- tion of 9. 81%- 12. 17% at each locus. 2 SNP sites associated with seed per silique were detected ,explaining the phe- notypic variation of 10. 44% and 10. 87% ,respectively. According to analyzing the gene annotation information of the LD region sequence associated with the SNP site,we predicted 16 candidate genes associated with seed density within per silique and its related traits. KMD4 and UGT-/6C2 genes are involved in the regulation of cytokinin. AGLI04 andADC2 were found to be related to seed formation. Other genes,such as MCCB,NGA2 and MATE,are known to be in- volved in the growth and development of lateral organs in a manner of abnormal expression. Two candidate genes ADC2 and UGT76C2,which overlapped in seed density within per silique and seed per silique,tended to have pleiotropism.
作者
任义英
崔翠
王倩
徐新福
林呐
殷家明
唐章林
李加纳
周清元
REN Yi-ying, CUI Cui, WANG Qian,XU Xin-fu, L1N Na, YIN Jia-ming, TANG Zhang-lin, LI Jia-na, ZHOU Qing-yuan(College of Agronomy and Biotechnology, Southwest University, Chongqing 400715)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2018年第2期314-325,共12页
Journal of Plant Genetic Resources
基金
国家科技支撑项目(2013BAD01B03-12)
国家农业部现代农业产业技术体系(CARS-13)
重庆市社会事业与民生保障科技创新(cstc2016shmszx0756)
西南大学博士启动基金(swu113064)
关键词
甘蓝型油菜
籽粒着生密度
连锁不平衡
基因组关联分析
Brassica napus L
seed density within per silique
linkage disequilibrium
genome-wide association a-nalysis
