摘要
目的探讨CD64、PCT及CRP在儿童ALL化疗后合并细菌感染早期诊断中的意义。方法收集本院儿童血液科2015年1月-2016年12月ALL化疗后合并细菌感染患儿75例,ALL化疗后未感染患者54例,同期健康体检儿童35例,采用流式细胞术检测外周血中性粒细胞CD64表达水平、固相免疫色谱法和速率散射比浊法测定血清中的PCT及CRP的含量。结果 ALL化疗后感染组CD64指数、PCT和CRP均明显高于ALL化疗后未感染组及健康对照组,差异有统计学意义(P<0.05)。当CD64荧光指数截断值为5.53时,其诊断灵敏度为90.67%,特异度为98.15%,均优于CRP、PCT单独诊断效率;当CD64指数、PCT及CRP3者联合使用时,灵敏度为98.67%,而特异度升高为98.15%。结论CD64指数、PCT及CRP联合检测能够提高对儿童ALL化疗后合并细菌感染早期诊断的敏感度和特异度。
Objective To investigate the significance of CD64,PCT and CRP in the early diagnosis of bacterial infection after chemotherapy in children. Methods 75 children with bacterial infection after ALL chemotherapy were enrolled in our hospital from January 2015 to December 2016. 54 patients with uninfected ALL after chemotherapy and 35 healthy children were enrolled in this study. The level of CD64 expression in peripheral blood neutrophils by flow cytometry,and the contents of PCT and CRP in serum were detected by solid phase immunosorbent assay and rate nephelometry. Results The CD64 index,PCT and CRP of ALL group were significantly higher than those of uninfected group and healthy control group after ALL chemotherapy,with the differences statistically significant（ P〈0. 05）. When the cutoff value of CD64 was 5. 53,the diagnostic sensitivity was90. 67% and the specificity was 98. 15%,which was better than CRP and PCT alone. When the CD64 index,PCT and CRP were used in combination,the sensitivity was 98. 67%,while the specificity increased to 98. 15%. Conclusion CD64 index,PCT and CRP combined test can improve the sensitivity and specificity of early diagnosis of bacterial infection in children after ALL chemotherapy.
作者
施灵敏
徐智胜
SHI Ling - min , XU Zhi - sheng(Pediatric Department, the Second Affiliated Hospital and Yuying Children＇s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, Chin)
出处
《中国卫生检验杂志》
CAS
2018年第4期467-469,共3页
Chinese Journal of Health Laboratory Technology
