摘要
目的探讨肛瘘患者Th1/Th2细胞因子平衡状态及其临床意义。方法应用微量样本多重蛋白定量技术(CBA)检测49例肛瘘患者外周血清Th1/Th2细胞因子,分析各细胞因子间相关性与临床特征的关系。结果肛瘘患者外周血清IL-4水平明显低于健康体检者,差异有统计学意义(P<0.01),TNF-α水平与健康体检者比较,差异无统计学意义(P>0.05),IL-4/THF-α明显低于健康体检者,差异有统计学意义(P<0.05)。肛瘘患者IL-10与TNF-α呈正相关(r=0.36,P<0.01),其他细胞因子无明显相关性(P>0.05)。肛瘘脓液细菌阳性的患者IL-4/TNF-α值低于细菌培养阴性的患者,差异有统计学意义(P<0.05)。结论肛瘘患者外周血清细胞因子以Th1细胞免疫反应为主,细菌培养阳性肛瘘患者Th1细胞免疫反应更为明显。
Objective To investigate the balance state of Th1/Th2 cytokines in patients with anal fistula and their clinical significance. Methods The serum levels of Th1/Th2 cytokines of 49 patients with anorectal fistula were detected by cytometric bead array technology（ CBA）. The relation between serum levels of cytokines and clinical characters were also analyzed.Results The IL-4 level of patients with anal fistula was significantly lower than the health controls,with the difference statistically significant（ P〈0. 01）; while the level difference of TNF-α was not statistically significant compared with the health controls（ P〈0. 05）. Moreover,the ratio of IL-4/TNF-α was significantly lower than the health controls,with the difference statistically significant（ P〈0. 05）. The serum level of IL-10 in patients with anal fistula was positively correlated with TNF-α（ r = 0. 36,P〈0. 01）,but had no correlation with other cytokines（ P〈0. 05）. The ratio of IL-4/TNF-α was also lower in patients with positive bacterial culture compared to patients with negative bacterial culture,with the difference statistically significant（ P〈0. 05）. Conclusion Patients with anal fistula presenting Th1 cells immune response was dominant,especially in patients with positive bacterial culture.
作者
林湘涛
方可欣
华国花
王永杰
竺王玉
LIN Xiang - tao , FANG Ke - xin, HUA Guo - hua, WANG Yong - jie, ZHU Wang - yu(Department of Anal -colorectal Surgery, Zhoushan Hospital, Zhoushan, Zhejiang 316021, Chin)
出处
《中国卫生检验杂志》
CAS
2018年第5期548-551,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省科技厅分析测试项目(2015C37013)
