摘要
为研究防治鸡大肠杆菌病的新方法,减少抗生素的使用及耐药菌株的产生,试验选取黄连、乌梅、白术等中药制成黄连组方口服液,检测其对大肠杆菌O_(78)的抑制作用与临床疗效。体外试验采用最小抑菌浓度(MIC)与最小杀菌浓度(MBC)检测口服液抑菌效果;应用流式细胞仪观察口服液对细菌死亡率的影响;利用扫描电镜和透射电镜观察口服液对菌体形态与结构的影响;临床试验检测口服液对鸡大肠杆菌病的治疗效果及免疫器官指数的影响。结果显示,口服液对大肠杆菌的MIC为62.5mg/mL,MBC为250mg/mL;流式细胞仪检测可见口服液组大肠杆菌的死亡率为74.1%,空白对照组大肠杆菌死亡率为37.2%;扫描电镜下可见口服液处理组的菌体溢缩、断裂,形成许多残体;透射电镜下可见菌体变形,质壁分离现象。临床疗效显示,黄连组方治愈率为86.67%,而西药组治愈率为63.33%;空白对照组和西药组脾脏指数与模型组差异极显著(P<0.01),空白对照组、西药组、黄连组方高剂量组的法氏囊指数极显著高于模型组(P<0.01),模型组的胸腺指数显著低于其余各组(P<0.05)。本试验结果表明,黄连组方可使菌体发生变形、溢缩断裂、质壁分离等变化达到杀菌效果,并可通过提高动物体免疫力来防治雏鸡大肠杆菌病。
To study the new method for prevention and treatment of chicken colibacillosis and reduce the use of antibiotics and drug-resistant strains,Coptis chinensis,Fructus mume,Atractylodes and other traditional Chinese medicine were selected to constitute the Coptischinensis prescription oral liquid.The inhibitory effect and clinical efficacy of Coptis chinensis prescription oral liquidon on E.coli O_（78） were tested.MIC and MBC were detected to show the oral antibacterial effect in vitro test.The effects of oral liquid on bacterial mortality were observed by flow cytometry.Meanwhile the effects of oral liquid on the morphology and structure of the cells were observed by scanning electron microscopy and transmission electron microscopy,in vitro test.The effect of oral liquid on the treatment of colibacillosis in chickens and the index of immune organ in clinical trials were investigated.The results showed that MIC was 62.5 mg/mL,MBC was 250 mg/mL.The result of flow cytometry showed that the mortality rate of E.coli was 74.1%in the oral liq-uid group but it was 37.2%in blank control group.Scanning electron microscopy showed that the cells of the oral liquid treatment group were spilled and broken,and many debris were formed.Under the transmission electron microscope,the cell deformation and the wall separation were observed.Clinical efficacy showed that the cure rate was 86.67%in Coptis chinensis prescription,while the cure rate was 63.33%in the group administrated with norfloxacin.The spleen index of blank control group and western medicine group were extremely significantly different from that of model group（P〈0.01）.The bursa index of blank control group,western medicine group and Coptis chinensis high dose group were extremely significantly higher than that of model group（P〈0.01）.The thymus index of model group was significantly lower than that of other groups（P〈0.05）.In a word,Coptis chinensis prescription could make the bacteria deformation,constriction fracture and wall separation to achieve the role of sterilization.Meanwhile,Coptis chinensis could enhance animals immune function to prescription prevention and treatment of chicks with E.coli disease.
作者
于文会
李叔洪
姜晓文
陈亚楠
钟秋
黄慧
YU Wenhui , LI Shuhong, JIANG Xiaowen, CHEN Yanan, ZHONG Qiu, HUANG Hui(Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin 150030, Chin)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第3期814-821,共8页
China Animal Husbandry & Veterinary Medicine
基金
公益性行业(农业)科研专项(201403051)
关键词
黄连组方
大肠杆菌O78
抑菌
雏鸡
临床疗效
Coptis chinensis prescription
E. coli O78
bacteriostatic
chicks
clinical efficacy
