摘要
作者等继1986年首次证实山东省恙虫病流行之后,于1987年9～11月间,在流行区进行了病原分离.用小鼠分别自小盾纤恙螨(Leptotrombidium(L) scutellarae)、黑线姬鼠(Apodemus agrarius)以及恙虫病患者血液分离出8株恙虫病立克次体,分离阳性率分别为10、25和50%.所有分离物感染小鼠的肝脾均有3～5倍的扩大,其中3株仅血清抗体阳性而小鼠不发病;5株可使小鼠有规律地发病,并可以从病鼠组织中查见立克次体颗粒,但毒力有区别,其中3株症状不典型、发病晚,有2株感染小鼠接种后5～12天发病,症状典型,7～15天部分小鼠死亡(25～75%).用酶标染色法进行血清学鉴定,结果表明.分离株与普氏立克次体(E株)、Q热立克次体(06株)和斑点热立克次体(246株)无任何交叉反应,而与恙虫病立克次体(Gilliam株)呈阳性反应.初步认为,自山东分离的恙虫病立克次体可能是与Gilliam同一血清型.但山东恙虫病为秋冬型.
Isolation of R tsutsugamushi was carried out in Shandong Province in Sept to Nov,1987.8 isolates were obtained from chiggers,wild rats and suspected patients,the positive rates were 10,25 and 50% respectively.Enlargement of liver and spleen was observed in all experimental mice inoculated with 8 strains,5 strains made the mice ill and two caused death.Serological identification of 8 isolates revealed strong positive reaction with R tsutsugamushi (Gilliam strain) and negative reaction with other groups of rickettsiae,including R prowazeki (Madrid E),C burnetti (Henzerling strain) and R sibirica (No 246).Thus,they were identified as R tsutsugamushi and suspected to be belonging to the Gilliam serotype.
出处
《军事医学》
CAS
CSCD
北大核心
1989年第1期43-46,共4页
Military Medical Sciences
基金
全军预防医学中心经费资助课题
关键词
恙虫病
立克次体
scrub typhus
isolation of R tsutsugamushi
identification of R tsutsugamushi
