摘要
目的同时建立肺癌原位移植瘤模型和异位皮下移植瘤模型,对比两种模型小鼠,以期发现它们在整体和肿瘤局部的生物学差异。方法将稳定表达虫萤光素酶的鼠Lewis肺腺癌细胞(ll2-luc-m38)与Matrigel胶的混悬液注射于C57 BL/6小鼠左肺建立肺癌原位移植瘤模型,与生理盐水混悬注射于C57 BL/6小鼠右侧腋后线皮下建立异位皮下移植瘤模型,定期利用小动物活体成像系统观察小鼠肿瘤形成及转移情况,记录小鼠生存时间,对小鼠肿瘤组织和肺部组织取材,石蜡包埋,切片,HE染色和免疫组化染色,做出病理学诊断。结果皮下移植瘤和肺原位移植瘤的成瘤率均为100%,肺原位移植瘤转移率为100%,小鼠出现了胸膜转移、心包转移、胸骨柄转移,其中1只小鼠出现了肾脏转移;皮下移植瘤转移率为70%,小鼠出现肺部转移。HE染色证实了两组均为肺腺癌,免疫组化染色结果示,肺原位移植瘤VEGF表达高于异位皮下移植瘤。结论肺癌原位移植瘤模型虽然操作复杂,小鼠生存期较短,但在转移率和肿瘤生物学特征更符合临床实际情况,研究者可根据不同实验需求选择合适的动物模型。
Objective To establish and compare an orthotopic non-small-cell lung cancer （NSCLC） mouse model and an ectopic murine model. Methods An orthotopic and an ectopic NSCLC model were established in C57 BL/6 mice through a injection of luciferase-expressing lewis ceils. The models were characterized by a survival study, histological staining, biolumines- cence imaging. Results The results demonstrated that cancer metastasis rate was significantly high in orthotopic model. Conclu- sion Furthermore,the expression of VEGF was higher compared to the ectopic model. It is concluded that the developed ortho- topic NSCLC model represents a feasible, reproducible, and clinically relevant experimental mouse model to test current and poten- tial therapies.
作者
马雪曼
王笑民
于明薇
张甘霖
杨国旺
于洁
MA Xueman, WANG Xiaomin, YU Mingwei, et al.(Beijing University of Chinese Medicine, Beijing, 10002)
出处
《实用癌症杂志》
2018年第4期523-526,共4页
The Practical Journal of Cancer
基金
国家自然科学基金(编号:81473643)
首都医科大学基础-临床科研合作基金(编号:15JL79)
北京市医院管理局青苗计划(编号:QML20150903)
关键词
肺癌
动物模型
原位移植法
异位移植法
Lung cancer
Animal model
Orthotopic murine model
Ectopic murine model
