摘要
目的:比较人牙髓干细胞(Dental pulp stem cells,DPSCs)与根尖牙乳头干细胞(Stem cells from the apical papilla,SCAPs)中mi RNAs的差异表达情况。方法:分离培养人DPSCs和SCAPs,标记STRO-1特异性抗体,利用免疫磁珠分选获得DPSCs和SCAPs。进行成骨样和成脂样细胞诱导分化,用碱性磷酸酶(ALP)、茜素红染色和油红O染色的方法鉴定其分化能力。采用二代测序技术,检测DPSCs和SCAPs中mi RNAs的表达,筛选差异表达的mi RNAs,运用生物信息学方法对差异表达mi RNAs进行靶基因预测和功能分析。结果:与DPSCs相比,SCAPs中表达下调超过1.5倍的mi RNAs有7个(hsa-mi R-224-5p、hsa-mi R-1247-5p、hsa-mi R-3065-3p、hsa-mi R-452-5p、hsa-mi R-767-5p、hsa-mi R-4284、hsa-mi R-146a-5p),无表达上调mi RNAs。这些表达下调的mi RNAs所调控的下游靶基因主要有27个,这些靶基因主要参与了细胞的迁移、分化和凋亡。结论:特定mi RNAs表达的下调以及其所调控的下游靶基因可能与SCAPs的干性维持相关。
Objective： To observe the differential expression of miRNAs between human dental pulp stem cells（DPSCs） and stem cells from the apical papilla（SCAPs）. Methods： DPSCs and SCAPs were isolated by immune-magnetic binding specific STRO-l antibody separation system. Osteogenic and adipogenic differentiation of DPSCs and SCAPs were tested by ALP assay, alizarin red staining（ARS） and Oil Red 0 staining. Differential miRNA expression of DPSCs and SCAPs was screened by the Next generation sequencing. Target genes and their possible roles of these differential miRNAs were predicted using biological information analysis. Results： The results revealed that 7 miRNAs（hsa-miR-224-5p, hsa-miR-1247-5p, hsa-miR-3065-3p, hsa-miR-452-5p, hsa- miR-767-5p, hsa-miR-4284, hsa-miR-146a-5p）were downregnlated while no miRNAs was upregulated in SCAPs compared with DPSCs. 27 target genes which mainly involved in the cell migration, differentiation and apoptosis were found. Conclusion： Down- regulation of some specific miRNAs might be related to the sternness of SCAPs.
作者
林诗晗
胡雪刚
吕红兵
LIN Shihan, HU Xuegang, LV Hongbing(350002 Fuzhou, Department of Endodontics, School and Hospital of Stomatology, Fufian Medical University, Chin)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2018年第2期239-243,共5页
Journal of Practical Stomatology
基金
福建省卫生系统中青年骨干人才培养项目(编号:2014-ZQN-ZD-22)
