摘要
从GenBank数据库下载犬瘟热病毒14种基因型H基因237株序列,与3株东北虎源犬瘟热病毒序列比对之后进行同源性和位点变异分析,最后构建系统发育树。研究发现:3株东北虎源犬瘟热病毒(KC579362、KC579363和KM386683)分别属于Arctic-like和Asia-1基因型,与其他基因型毒株的同源性为89.94%~100%之间,H基因序列1 632(G→A)、1 664(C→T)、1 729(G→A)和1 795(G→A)位点的突变可能是CDV跨宿主传播给东北虎的关键突变位点。Arctic-like和Asia-1型毒株对东北虎威胁最大,H基因变异位点可为研究东北虎源序列遗传变异特征提供分子生物学依据。
In this study, 237 strains of 14 genotypes of CDV were retrieved from the GenBank data- base, which were aligned with 3 strains in Amur tiger, then analyzed homology and mutation sites, and finally built a phylogenetic tree. The results showed that these 3 strains in Amur tiger （KC579362, KC579363 and KM386683 ） belonged to Arctic-like and Asia-1 genotypes, respectively, and had 89.94%-100% homology of gene sequence with other genotype strains. In the H gene sequence, the mutations in 1 632 （G→A）, 1 664 （C→T）, 1 729 （G→A） and 1 795 （G→A） sites may be the key mutation sites for CDV crossing the host to the Amur tiger. Arctic-like and Asia-1 genotypes have the greatest threat to the Amur tiger, and the H gene mutation sites can provide molecular biology basis for studying the genetic variation of CDV sequence in Amur tiger.
作者
江峰
王昊宁
黄利亚
王晓龙
JIANG Feng;WANG HaoningI;HUANG Liya;WANG Xiaolong(College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China;Center of Conservation Medicine ＆ Ecological Safety, Northeast Forestry University, Harbin 150040, China;Changbai Mountain Academey of Science, Antu 133613, China)
出处
《经济动物学报》
CAS
2018年第1期6-9,共4页
Journal of Economic Animal
基金
国家重点研发计划专项(2017YFD0501702)
关键词
犬瘟热病毒
东北虎
H基因
基因型
位点变异
canine distemper virus
Amur tiger
H gene
genotype
site mutation
