摘要
目的:研究大黄芪汤抑制化疗所致lewis肿瘤肺转移的作用及机制。方法:40只C57BL/6小鼠随机分为对照组、中药组、化疗组、联合中药组,每组各10只。对照组单予生理盐水腹腔注射,中药组用生理盐水腹腔注射联合大黄芪汤灌胃,化疗组小鼠予顺铂腹腔注射,联合中药组予顺铂腹腔注射联合大黄芪汤灌胃。化疗末次后4 d,对所有小鼠尾静脉注射lewis细胞。20 d后检测小鼠肺内转移病灶数、瘤重,并分析瘤周肺组织ATF3、瘤体B7-H3表达。结果:1.肺内转移病灶数:对照组、中药组、化疗组、联合中药组、分别为4.7、4.9、7.6、5.5个,化疗组与其余三组分别比较显著升高,P<0.01。2.瘤重:对照组、中药组、化疗组、联合中药组分别为1.41、1.47、1.92、1.43g,化疗组与其余三组分别比较显著升高,P<0.01。3.瘤周正常肺组织ATF3、瘤体B7-H3表达:化疗组均高于对照组,联合中药后其表达均降低。结论:大黄芪汤可以抑制化疗所致的lewis肿瘤肺转移,其机制可能与其降低正常肺组织ATF3及肿瘤组织B7-H3表达有关。
Objective： To investigate the inhibiting effects and mechanisms of DaHuangQi Decoction on lewis Lung Carcinom metas- tasis which was induced by chemotherapeutics. Methods： 40 C57BL/6 mice were random devided into Chemotherapy group, Chemo- therapy combined with Chinese medicine group, Chinese medicine group, Contral group （with each group 10 mice） . The chemotherapy group was intraperitoneal injected （ i. p. ） cisplatinion. The combined Chinese medicine group dealed with cisplatin i. p. and DaHuangQi Decoction gastric infusion （ i. g. ） . The Chinese medicine group was injected with physiological saline （NS） i.p. and Chinese medicine i. g.. The Contral group was given NS i. p. only. Four days later of the last chemotherapy, for all mice, lewis Lung Carcinom cells were injected via the tail vein. 20 days from tail vein injection, we will measure the tung metastatic lesions number, tumor weight, ATF3 which belong to normal lung tissue that around tumor and the tumor B7-H3＇ s expression were analyzed. Results： 1. The lung metastatic lesions number between The chemotherapy group, Chemotherapy combined with Chinese medicine group, The traditional Chinese medicine group, and The Contral group were 7. 6, 5.5, 4. 9 and 4.7 respectively. The comparison among the chemotherapy group and the other three groups, all of the P values are less than 0. 01.2. The tumor weight of four groups were 1.92, 1.43, 1.47 and 1.41 g respectively. The comparison among the chemotherapy group and the other three groups, all of the P values are less than 0. 01.3. The ATF3 that around tumor and tumor B7-H3＇s expression： Chemotherapy group was higher than that the Contral group, and the expression of the combined Chinese medicine was decreased. Condtmiorl： DaHuangQi Decoction can inhibit the metastasis of lewis Carcinom lung metastasis whieh caused by chemotherapy, and its mechanism may be related to the reduced expression of normal lung tissue ATF3 and tumor tissue B7-H3.
作者
熊绍权
李亚玲
罗秋月
吕盼盼
姚德祎
何成诗
XIONG Shao.quan, LI Ya-ling, LUO Qiu-yue, LV Pan-pan, YAO De-wei, HE Cheng-shi(Chengdu University of Traditional Chinese Medicine Hospital, Chengdu, 610072, Chin)
出处
《成都中医药大学学报》
2018年第1期16-19,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81573968)
四川省中医药管理局项目(2014A026)
四川省教育厅项目(13ZA0277)
