摘要
目的:观察大鼠视神经损伤后视网膜、视神经的病理变化,探讨血府逐瘀汤对其形态结构的影响。方法:36只大鼠分为正常组4只,治疗组16只(血府逐瘀汤灌胃),对照组16只(蒸馏水灌胃)。治疗组和对照组建立视神经损伤大鼠模型,造模后3d、1周、2周、4周取材,制作切片,分别用光镜、电镜观察各组视网膜形态学和视神经超微结构的变化。结果:(1)视网膜:造模后3d时对照组视网膜各层水肿增厚,神经纤维层最重,节细胞肿胀;造模1周、2周时对照组神经纤维层水肿减轻,节细胞大量减少,内核层细胞略减少,内、外丛状层水肿加重;4周时视网膜各层水肿减轻,对照组神经纤维层较治疗组萎缩变薄,治疗组节细胞密度较对照组高。(2)视神经:造模3d时对照组和治疗组髓鞘板层分离、变薄,轴突肿胀减少,部分微丝微管崩解消失;造模后1周时髓鞘板层分离加重,轴突降解,神经纤维变性形成"洋葱样"小体;造模后2周时对照组和治疗组髓鞘薄膜样,变性轴突消失,胶质细胞增生;造模后4周时对照组和治疗组超微结构较前均有修复,治疗组髓鞘结构较对照组增厚、致密,轴突电子密度高,微管、微丝数目明显增多,线粒体结构清晰。胶质细胞接近正常。结论:视网膜、视神经在外伤后有一定的自我修复规律;血府逐瘀汤能够减轻视网膜、视神经结构损坏。
To observe the pathological changes of retina and optic nerve of the optic nerve injury rats,and to explore the effect of Xuefu Zhuyu Decoction on the morphology and structure of the retina. Methods： Thirty-six rats were divided into normal group（n= 4）,treatment group（n =16） and control group（n =16）. The rat optic nerve injury rat model was established in the treatment group and the control group,and 3 days,1 week,2 weeks,4 weeks after the model establishment,the slices were made,the changes of retinal morphology and optic nerve ultrastructure were observed by light microscope and electron microscope respectively. Results：（1） Retina： The 3rd day： Each layer of retina,especially the nerve fiber layer edema increased. The ganglion cells became swollen. The 1 st and 2 nd week： The thickness of nerve fiber layer decreased with edema reducyion. A large number of ganglion cells decreased,and the inner nuclear cells decreased slightly. The inner plexiform layer and the outer plexiform layer edema increased. The 4 th week： Each layer of retina edema reduced. The nerve fiber layer of Control Group was thinner than that of Treatment Group,and the ganglion cell density of Treatment Group was higher than that of Control Group.（2）Optic nerve： The 3rd day：Lamellar separation of myelin can be seen. The axons became swollen and reduced. The numbers of microfilaments and microtubules were both reduced significantly. The 1 st week： Lamellar separation of myelin can be seen further expansion than before. Parts of nerve fibers degradated and formed ＂onion-like body＂. The 2 nd week： The myelins bacame thiner. Some axons disappeared. The glial cells proliferated. The 4th week： The ultrastructures of optic nerve in the two groups were all repaired. Compared with Control Groups,the lamellar structure of myelin of Treatment Group repaired obviously. A large number of axons gradually restored,and the electron density increased. The numbers of microtubule,microfilament increased. The mitochondria structure was clear. The glial cells were almost normal. Conclusion： Retina and optic nerve can repair itself after trauma. Xuefu Zhuyu Decoction can reduce the retina and optic nerve structure damage.
作者
崔红培
崔龙江
庄曾渊
李莉
CUI Hongpei1, CUI Longjiang1 , ZHUANG Zengyuan2, LI Li3(1. Henan Eye Provincial Hospital,Zhengzhou Henan China 450008 ; 2. Eye Hospital of Academy of Chinese Medicine Science, Beijing China 100040; 3. Research Institution of Orthopaedics of China Academy of Chinese Medicine Science, Beijing China 10070)
出处
《中医学报》
CAS
2018年第3期430-435,共6页
Acta Chinese Medicine
关键词
血府逐瘀汤
视神经损伤
视网膜
视神经
形态结构
小鼠
Xuefu Zhuyu Decoction
optic nerve injury
retina
optic nerve
morphological structure
rat
