摘要
目的研究白花蛇舌草多糖提取物(HDP)对人喉癌Hep-2细胞的抗瘤作用。方法采用MTT测量细胞活性,流式细胞仪进行凋亡分析。Transwell培养小室进行细胞侵袭实验,Western blotting测量多糖提取物诱导凋亡和抗侵袭活性的机制。结果 HDP对喉癌细胞的抑制呈时间和浓度依赖性,浓度越高,时间越长抑制率越高。细胞周期分析发现HDP(400μg/m L)使细胞滞留于G0/G1期,同时发现药物作用24 h后细胞凋亡率明显升高,Western blotting结果分析发现相关凋亡蛋白caspase-3,caspase-8和caspase-9表达升高,而Bcl-2表达下降。同时细胞侵袭实验还发现HDP抑制喉癌细胞的侵袭,抑制M M P-2和u PA相关蛋白的表达。结论研究发现HDP抑制喉癌细胞的凋亡和侵袭,对恶性肿瘤的治疗有潜在的应用价值。
Objective The aim of this study was to explore the anti-tumor potential of a polysaccharide isolated from Hedyotis diffusa（ HDP） in Hep-2 human larynx squamous carcinoma cells. Methods Cell cycle and apoptosis were analyzed by detecting changes in protein expression by flow cytometry. Apoptosis was further characterized by detecting relevant proteins by western blotting.Results HDP suppressed the proliferation of Hep-2 cells in a time-and dose-dependent manner. Cell cycle analysis revealed that exposure to HDP（ 400 μg/mL） caused G0/G1 cell cycle arrest in Hep-2 cells. Moreover,flow cytometry revealed that Hep-2 cells treated with HDP for 24 h underwent apoptosis to a significantly greater extent than untreated control cells. HDP treatment promoted the expression of caspase-3,caspase-8,and caspase-9,and reduced the expression of Bcl-2. HDP also inhibited cell migration and suppressed the expression of MMP-2 and uPA. Conclusion HDP possesses suppressive effects against aggressive phenotypes of human larynx squamous carcinoma cells and may have therapeutic potential for this particular malignancy.
作者
赵小燕
吴彩琴
任晓勇
王正辉
ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui(Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xi＇an Jiaotong University, Xi＇an 710004, Shaanxi, Chin)
出处
《山东大学耳鼻喉眼学报》
CAS
2018年第2期84-87,共4页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
陕西省科技攻关项目(2015SF044)
关键词
白花蛇舌草
多糖
HEP-2细胞
凋亡
侵袭
Hedyotis diffusa
Polysaccharide
Hep-2 Ceils
Apoptosis
Migration
