摘要
目的对石家庄市2007-2013年金黄色葡萄球菌食物中毒株进行分子分型、肠毒素检测和耐药性分析,了解金黄色葡萄球菌食物中毒株的遗传学特征。方法利用多位点序列分型(MLST)技术对食物中毒事件中检出的金黄色葡萄球菌进行分子分型,采用金黄色葡萄球菌肠毒素ABCDE分型检测试剂盒对分离的金黄色葡萄球菌进行葡萄球菌肠毒素分型,采用BD Phoenix?生化药敏鉴定仪对分离株进行药敏检测。结果 47株食物中毒株金黄色葡萄球菌分14个MLST型,分别为:ST15、ST59、ST5、ST398、ST6、ST1003、ST88、ST30、ST338、ST464、ST7、ST72、ST804和ST9。葡萄球菌肠毒素类型分别为SEE,SEA-SEB-SEC,SEA-SED-SEE,SEB-SEC,SEC-SED-SEE,SEA-SEE和SEASEB-SED-SEE。93.6%(44/47)的食物中毒株产β-内酰胺酶,97.9%(46/47)的分离菌株对青霉素耐药,对其他11种受试药物表现出不同程度的敏感,少数菌株表现为耐药。结论多个MLST型的金黄色葡萄球菌均可引起食物中毒,食物中毒株可产多种葡萄球菌肠毒素,对多种抗菌药物敏感。
Objective Objective To study the characteristics of Staphylococcus aureus strains isolated from outbreaks of food poisoning from 2007 to 2013 through genotyping,staphylococcal enterotoxin testing,and analysis of its drug resistance. Methods A multi-locus sequence typing（MLST）system was used to analyze S.aureus strains from outbreaks of food poisoning.Staphylococcal enterotoxins were detected with ELISA using a Ridascreen SET A,B,C,D,E assay kit.Drug susceptibility testing was also performed using the BD Phoenix Automated Microbiology system. Results In 47 S.aureus strains,14 MLST types were identified：ST15,ST59 ST5,ST398,ST6,ST1003,ST88,ST30,ST338,ST464,ST7,ST72,ST804,and ST9.Seven staphylococcal enterotoxin types were identified：SEE,SEA-SEBSEC,SEA-SED-SEE,SEB-SEC,SEC-SED-SEE,SEA-SEE,and SEA-SEB-SED-SEE.Of the S.aureus isolates examined in this study,93.6%（44/47）produced beta-lactamases and 97.9%（46/47）were resistant to penicillin.Isolates were not resistant to amikacin,teicoplanin,linezolid,nitrofurantoin,rifampin,or vancomycin.A few strains were resistant to several types of drugs. Conclusion Numerous MLST types of S.aureus caused food poisoning and they produced a variety of staphylococcal enterotoxins.The S.aureus strains causing outbreaks of food poisoning were sensitive to many antibiotics.
作者
吕国平
李亚子
郭玉梅
任兴烨
陈雅萍
秦丽云
李丽婕
方鹏
仵立佳
LV Guo-ping, LI Ya-zi, GUO Yu-mei, REN Xing-ye, CHEN Ya-ping, QIN Li-yun, LI Li-jie, FANG Peng, WU Li-jia(Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050000, Chin)
出处
《中国病原生物学杂志》
CSCD
北大核心
2018年第2期185-188,共4页
Journal of Pathogen Biology
