摘要
对壮骨止痛方有效成分进行关联分析,探讨其对"肾虚血瘀证"疾病网络的干预作用。通过对壮骨止痛方传统功效、有效成分和现代药理作用研究的文献调研,并利用Cytoscape软件进行复杂网络分析,同时还验证了壮骨止痛方组方结构,并有效地归纳了有效成分的"君、臣、佐、使"。壮骨止痛方药效物质基础为补骨脂素、淫羊藿苷、枸杞多糖、骨碎补总黄酮、齐墩果酸、金粉蕨素和牛膝甾酮。壮骨止痛方的补肾活血功效与其提高雌激素、增加骨密度等现代药理作用有较大的关联性。壮骨止痛方的有效成分符合各单味药"君、臣、佐、使"的组方结构,壮骨止痛方其有效成分可经由Wnt/β-catenin、ERK/MAEK/NF-κB等多条信号通路相互作用,多途径、多靶点共同发挥补肾活血壮骨作用。
To analyze the intervention of the effective components of Zhuanggu Zhitong Prescription for the disease networks of blood stasis syndrome of kidney deficiency. According to the literature research on the traditional efficacy, active ingredients, and modern pharmacology of Zhuanggu Zhitong Prescription, the complex network analysis was performed by using cytoscape, and the structure of Zhuanggu Zhitong Prescription was also validated. The results showed that the effective constituents of Zhuanggu Zhitong Prescription were psoralen, epimedium glycosides, lycium barbarum polysaccharides, total flavonoids of Rhizoma Drynariae, oleanolic acid, fennelin, and inocosterone. Moreover, four basic elements of Sovereign-Minister-Assistant-Guide（Jun-Chen-Zuo-Shi） of the effective components of Zhuanggu Zhitong Prescription were summarized effectively. There have a greater correlation between tonifying kidney and activating blood function of Zhuanggu Zhitong Prescription and modern pharmacology, such as raising estrogen levels and increasing the degree of bone mineral density. The effective ingredients in Zhuanggu Zhitong Prescription in line with the prescription structure of Sovereign-Minister-Assistant-Guide in the single herb medicine. The active ingredients of Zhuanggu Zhitong Prescription can interact with multiple signaling pathways through multi-channel and multi-target such as Wnt/β-catenin, ERK/MAEK/NF-k B and so on, in order to exert the effect of tonifying kidney and activating blood and strengthening bone.
作者
刘乐平
刘慧萍
谢瑾如
杨璐瑜
王梦雅
彭婷
刘平安
张国民
LIU Le-ping, LIU Hui-ping, XIE Jin-ru, YANG Lu-yu, WANG Meng-ya, PENG Ting, LIU Ping-an, ZHANG Guo-min(Key Laboratory of Translational Medicine in the Research of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, Chin)
出处
《中草药》
CAS
CSCD
北大核心
2018年第6期1451-1460,共10页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(81303123
81573956)
湖南省中医药科研计划重点项目(201612)
湖南省教育厅科学研究重点项目(16A162)
国家级大学生创新创业训练计划项目(201710541002)
湖南省大学生研究性学习和创新性实验计划项目(2015217
2015220
2016284
2016281)
湖南省自然科学基金面上项目(2018JJ2297)
关键词
壮骨止痛方
有效成分
关联分析
传统功效
补肾活血
补骨脂素
淫羊藿苷
枸杞多糖
齐墩果酸
Zhuanggu Zhitong Prescription
active ingredients
association analysis
traditional efficacy
tonifying kidney andactivating blood
epimedium glycosides
lycium barbartma polysaccharides
oleanolic acid
