摘要
目的:观察单纯脑震荡及多重脑震荡对大鼠空间认知行为的远期影响。方法:7周龄SD雄性大鼠180只,体重280±30 g,随机分为实验对照组(C)和脑震荡组,用单摆打击装置复制大鼠脑震荡模型。脑震荡组完成第一次打击后随机分为单纯脑震荡(PCC)组和多重脑震荡(MCC)组,多重损伤组完成第二次打击后再随机分为二重脑震荡组(2MCC)和三重脑震荡组(3MCC),重复脑震荡模型打击间隔时间为24 h,对伤后1月、3月、6月大鼠进行水迷宫实验评估其空间认知功能改变。结果:1.伤后1月:(1)逃避潜伏期:与对照组比较,PCC组无统计学差异,2MCC组于第7天,3MCC组于第6、7天有统计学差异。与PCC组比较,2MCC组于第7天,3MCC组于第6、7天有统计学差异。2MCC组与3MCC组比较于第7天有统计学差异。(2)无平台探测实验:各组间差异无统计学意义。2.伤后3月:(1)逃避潜伏期:与对照组比较,PCC组无统计学差异,2MCC组于第5、6、7天,3MCC组于第4、5、6、7天延迟有统计学意义。与PCC组比较,2MCC组无统计学差异,3MCC组第6、7天有统计学差异。2MCC组与3MCC组比较无统计学差异。(2)无平台探测实验:与对照组比较,PCC无统计学差异,2MCC组和3MCC组停留时间缩短具有统计学意义。与PCC组比较仅3MCC组具有显著统计学意义。2MCC组与3MCC组比较无统计学差异。3.伤后6月:(1)逃避潜伏期:与对照组比较,PCC组于第6、7天有统计学差异,2MCC组于第5、6、7天,3MCC组于第2、3、4、5、6、7天延迟有统计学意义。与PCC组比较,2MCC组于第6、7天,3MCC组于第4、5、6、7天有统计学差异。2MCC组与3MCC组比较于第7天有统计学差异。(2)无平台探测实验:与对照组比较,PCC组、2MCC组和3MCC组在原平台象限停留时间缩短均有统计学差异;与PCC组比较,2MCC组和3MCC组具有显著统计学意义,2MCC组与3MCC组比较无统计学差异。结论:随着脑震荡次数的增加,大鼠出现空间认知行为较早和较严重的损坏,并有明显的损伤累积效应,该模型可用于慢性创伤性脑病病理改变的研究。
Objective To detect the long term effect of pure and multiple concussions on spatial cognitive of rats. Methods One hundred and eighty 7-week-old Sprague-Dawley male rats with weight of 280 ± 30 g were chosen and randomly divided into a control group and a concussion group. The cerebral concussion was induced in the rats using a metallic pendulum striker concussive device. After the first strike, the brain injury group was randomly divided into a pure cerebral concussion（PCC）group and a multiple cerebral concussion（MCC） group. After the second strike, the MCC group was randomly divided into two-fold cerebral concussion（2 MCC） group and three-fold cerebral concussion（3 MCC）group. The striking interval was 24 h. One, 3 and 6 months after trauma, their cognitive function was tested using Morris water maze. Results One month later after injury, there was no significant difference in the escape latency between the control group and PCC group. Significant differences in the measurement were observed between the control/PCC group and 2 MCC group on the 7 th day after the injury, also between the control/PCC and 3 MCC groups on the 6 th and 7 th day. And there were significant differences between the 2 MCC and 3 MCC groups on the 6 th and 7 th days. The non-platform testdid not observe any significant differences among the four groups. Three months after injury, there wasstill no significant difference between the control group and PCC group, PCC and 2 MCC groups, aswell as 2 MCC and 3 MCC groups in the escape latency. However, there was significant difference between the control group and 2 MCC group on the 5 th, 6 th and 7 th days, between the control group and3 MCC group on the 4 th, 5 th, 6 th and 7 th days, as well as between PCC group and 3 MCC group onthe 6 th and 7 th days. In the non-platform test, there was no significant difference between the control group and PCC group, between PCC group and 2 MCC group, as well as between 2 MCC group and 3 MCC group. However, 2 MCC and 3 MCC groups spent significantly less time in the former platformquadrant, when compared with the control group and 3 MCC group spent significantly less time thanPCC group. Six months after injury, significant differences in the escape latency were observed between the control group and PCC group on the 6 th and 7 th days, and 2 MCC group on the 5 th, 6 th and7 th days, also and 3 MCC groups on the 2 nd, 3 rd, 4 th, 5 th, 6 th and 7 th days, still between PCCgroup and 2 MCC group on the 6 th and 7 th days, as well as between PCC group and 3 MCC group onthe 4 th, 5 th, 6 th and 7 th days. Moreover, there was significant difference between 2 MCC and 3 MCCgroups only on the 7 th day. In the non-platform test, PCC group, 2 MCC group and 3 MCC group spent significantly less time in the former platform quadrant compared with the control group. Moreover, in this test significant differences were found between PCC group and 2 MCC/3 MCC group, but not between 2 MCC group and 3 MCC group. Conclusion With the increase of cerebral concussion times,earlier and more serious damage of spatial cognition will appear, with a significant cumulative effect in rats. Such rat model can be used to study the pathological changes of cognitive impairment in chronic traumatic encephalopathy.
作者
王家雄
王路
宋海
郑永江
张睿
张桓
杨力
郭泽云
于建云
Wang Jiaxiong2,4, Wang Lu1, Song Hal1,2, Zheng Yongjiang1, Zhang Rui1, Zhang Huan1, Yang Li3, Guo Zeyun3, Yu Jianyun1(1 Forensic Medical College, Kunming Medical University, Kunming 650500, China 2 Neurosurgery Department of the First Affiliated Hospital of Kunming Medical University, Kunming 650500, China 3 Department of Histology and Embryology, Kunming Medical University, Kunming 650500, China 4 Department of Neurosurgery, No. 1 People＇s Hospital of Honghe Prefecture, Honghe 661100, Chin)
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2018年第3期218-223,共6页
Chinese Journal of Sports Medicine
基金
国家自然科学基金项目(81360467)
云南省自然科学基金应用基础联合专项重点项目(2013FB104)
关键词
多重脑震荡
水迷宫
认知行为
慢性创伤性脑病
大鼠
multiple cerebral concussion, morris water maze, cognitive behavior, chronic traumaticencephalopathy, rats
