摘要
目的综合改良模型构建方法,以期缩短建立大鼠放射性颌骨坏死(ostecoradionecrosis of jaw,ORNJ)模型的时间,并通过临床大体、病理及影像学对其进行动态评价。方法健康SD雄性大鼠随机分为1组空白对照组(8只)和4组照射组(每组6只)。使用改良大鼠固定和防护照射装置,通过X线生物学辐照器单次7 Gy,每天1次,连续5天对大鼠左侧下颌骨进行精准照射。在照射后第1周内所有大鼠高蛋白饮食,在照射后第7天使用改良拔牙法彻底拔除所有大鼠左侧下颌3颗磨牙,于第7、14、21、28天分别处死2只空白对照组大鼠和1组照射组大鼠,进行临床大体观察、组织病理学检查、Micro-CT扫描等。使用SPSS18.0软件包对数据进行统计学分析。结果通过28天的实验过程成功建立大鼠ORNJ模型,同时建模成功率提高至83%,且照射组大鼠存活率达到100%。动态观察:大体可见照射组大鼠在照射后第7天出现左侧颊部黏膜溃疡,随后逐渐出现照射区脱毛、咬合紊乱、颊部溢浓等;且该组大鼠照射后体重持续下降,至第14天才逐渐上升。病理骨髓腔的改变先于皮质骨。在照射后第7天骨髓腔内脂肪空泡增多,随后出现骨髓坏死,并持续加重。皮质骨第7天即可观察到空白骨陷窝增多,第21天纤维组织明显增多,随后坏死骨组织被增生的纤维组织取代。影像学第28天可见皮质骨明显缺损。结论短期内建立的大鼠ORNJ改良模型高效且稳定可靠,在明显缩短建模周期的同时,又可全面反映ORNJ在临床大体、病理、影像学上的动态改变,因此是用于研究该疾病发病机制及早期防护干预措施较为理想的动物模型,值得进一步研究和推广。
OBJECTIVE To establish an modified model of osteonecrosis in the rat and observe the clinical, pathological and imaging manifestations dy namically. METHODS Healthy male SD rats were divided into 5 groups randomly, including the control group（N, 8 rats）, and radiation groups（a,b,c and d, 6 rats each g roup）. For radiation groups, the left mandible of each rat was irradiated at doses of 7.0 Gy for 5 fractions, other rats were sham irradiated, serving as control groups. 7 days after irradiation, the left mandibular molars of all rats were extracted. The radiation groups rats were sacrificed at 7, 14, 21, 28 days after irradiation randomly, the 2 rats in control group were sacrificed respectively. All the rats＇ mandibles were taken and examined by clinical manifestation and Micro-CT and histology methods. The data was analyzed with SPSS 18.0 software package. RESULTS In clinical observation, ulcers were seen in the irradiated site at the 7 th day, after then, alopecia, occlusion disorder, and pus discharged from buccal side were seen. Weight loss rightly after radiation and weight gain were seen at the 14 th day. In pathological observation, marrow cavity changed first. Many adipose cell was first seen at the 7 th day, and inflammations, necrosis, and fibrosis were found in medulla in the irradiated groups after that. Significant increment of empty lacunae and decrement of osteocytes were observed in all irradiated groups at the 7 th day. Necrotic bone of the irradiated mandible was only found at the 28 th day. Lack of new bone formation within the socket was seen in the Micro-CT threedimensional at 28 th day, and there are no more changes at the early-stage in this model. CONCLUSION An modified rat model of osteoradionecrosis was successfully established, which can shorten experimental period, and this model can be used to observe clinical, pathological and imaging magnification, which play a basic role in the protection in early stage and deserved to be further researched.
作者
李洁
孔祥波
刘仰
陈雪英
钟婉珍
房思炼
LI Jie1, KONG Xiangbo2, LIU Yang1, CHEN Xueying1, ZHONG Wanzhen1, FANG Silian1(1 Department of Oral and Maxillofacial Surgery, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou,Guangdong, 510655, China; 2 Department of Stomatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, Chin)
出处
《中国耳鼻咽喉头颈外科》
CSCD
2018年第3期115-119,共5页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
广东省自然科学基金(2015A030313064)
广东省科技计划项目(2014A020212127)联合资助
关键词
模型
动物
评价研究
放射性颌骨坏死
Models, Animal
Evaluation Studies
osteoradionecrosis
