摘要
目的了解矽尘对作业工人叉头转录因子3(forkhead/winged helix transcription factor,FOXP3)基因启动子区域DNA甲基化状态的影响。方法选取健康检查者和矽尘作业工人为对照组和接尘组,采用亚硫酸氢盐测序法检测外周血FOXP3基因启动子特定区域DNA甲基化水平。结果在男性中,无论对照组还是接尘组FOXP3基因启动子区(+2071,+2182)5个Cp G位点均呈现高甲基化(100%)。在女性中,对照组第1个Cp G位点(+2102)和第4个Cp G位点(+2144)呈现低甲基化(40%),而接尘组相关Cp G位点可见高甲基化(100%)。结论女性接尘作业工人FOXP3基因启动子特定位点DNA高甲基化可能与矽尘的毒性有关。
[Objective]To investigate the effect of silica dust on DNA methylation in the promoter of transcription factor forkhead box protein 3（FOXP3） gene in the exposed workers.[Methods] Then the level of DNA methylation in the particular region of the FOXP3 gene promoter was in the peripheral blood detected by bisulfite sequencing.[Results] In males,hypermethylation（100%）was observed in 5 Cp G sites of FOXP3 gene promoter（＋2071,＋2182） in the control group as well as in the exposure group.In females,the first Cp G site（＋2102）and the fourth Cp G site（＋2144）were found to be hypomethylated（40%） in the control group,while those in the exposure group were hypermethylated（100%）.[Conclusion] The hypermethylation in the particular sites of the FOXP3 gene promoter might be relevant with the toxicity induced by silica dust in the female workers.
作者
廖静
李小峰
王春香
LIAO Jing1, LI Xiao-feng2, WANG Chun-xiang3(1.Occupational Disease Prevention and Control Department,Shenzhen Longgang District Center for Disease Control and Prevention,Shenzhen Guangdong,518172,China;2.Clinical Laboratory,Longgang District People＇s Hospital of Shenzhen, Shenzhen Guangdong, 518172,China;3.Occupational Health Department,Buff Health Care Center of Longgang District,Shenzhen Guangdong, 518112, Chin)
出处
《职业与健康》
CAS
2018年第2期162-166,共5页
Occupation and Health
基金
深圳市科技计划项目(JCYJ20150402164314696)
深圳市龙岗区科技发展资金(YLWS20150513174008294)
深圳市龙岗区科技发展资金(YLWS20150511114456591)
深圳市卫生计生系统科研项目(201402126)
