摘要
目的 探讨黄芪总苷对染镉大鼠免疫功能及酶活性的影响。方法 将36只大鼠随机均分为空白组、模型组、黄芪总苷组。末次给药24 h后处死大鼠,计算胸脾指数。MTT法测定脾淋巴细胞的转化功能和NK细胞的杀伤活性,比色分析法测定大鼠脾脏中SOD、MDA的活性及大鼠血清中AST、ALT、LDH的活性,用ELISA法测定大鼠脾脏组织中IL-2、TGF-β1的含量。结果 与空白组比较,模型组的体重、胸脾指数、SOD的活性、IL-2的含量、淋巴细胞转化能力和NK细胞杀伤能力均明显降低;但模型组MDA、TGF-β1的含量及AST、ALT、LDH的活性均明显升高。与模型组比较,黄芪总苷组的体重、脾脏指数、SOD的活性、IL-2的含量、淋巴细胞转化能力和NK细胞杀伤能力明显升高,而MDA、TGF-β1的含量及AST、ALT、LDH的活性均明显降低。结论 黄芪总苷对镉所致大鼠的酶活性和免疫损伤具保护作用。
OBJECTIVE To study the effects of astragalosides on the immune function and enzyme activity in rats induced by cadmi- um. METHODS 36 SPF Wistar rats were randomly divided into three groups ： Blank group, cadmium - exposed group, and astragalo- sides group. After rats were sacrified, the indexes of thymus and spleen were calculated by weighing method. The transformation ability of T lymphocyte and the killing activity of NK cells were measured by MTT. The levels of SOD, AST, ALT, LDH and MDA were detected by colorimetrie assay. The contents of IL -2 and TGF -β1 were analyzed by ELISA. RESULTS Compared with the blank group, the weight and the indexes of thymus and spleen were decreased significantly in the cadmium - exposed group, the SOD activity and the IL- 2 content were also decreased significantly in the group. Meantime, the transformation ability of T lymphocyte and the killing activity of NK cells were reduced obviously in the cadmium - exposed group, while the levels of MDA,TGF - 131 , AST, ALT, LDH were increased. Compared with the cadmium - exposed group, the weights and the spleen index were both risen significantly in astragalosides treated group and the SOD activity and the IL -2 content were also improved significantly. The transformation ability of T lymphocyte and the killing activity of NK cells were enhanced obviously in astragalosides treated group. Yet the levels of MDA, TGF -β1, AST, ALT,LDH were lowered obviously in astragalosides treated group. CONCLUSION Astragalosides has protective effect on the immu- nity function damage and enzyme activity induced by cadmium in rats.
作者
颜春鲁
安方玉
刘永琦
金华
伍志伟
苏韫
高军太
黄钰芳
YAN Chunlu1 , AN Fangyu1 , LIU Yongqi1 , JIN Hua1 , WU Zhiwei1 , SU Yun1 , GAO Juntai2, HUANG Yufang1(1. Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Traditional Chinese Medicine,Lanzhou, Gansu ,730000 P.R. China;2. Chinese Medicine Hospital of Pingliang , Pingliang , Gansu, 744000 P. R. Chin)
出处
《华西药学杂志》
CAS
CSCD
2018年第2期171-174,共4页
West China Journal of Pharmaceutical Sciences
基金
甘肃省自然科学基金项目(1508RJZA046)
甘肃中医药大学中青年基金项目(ZQ2014-9)
甘肃中医药大学教学研究与教学改革项目(ZH-201606)
甘肃省高等学校科研项目协同创新团队(2017C-14)
关键词
黄芪总苷
镉染毒
酶活性
免疫功能
淋巴细胞
NK细胞
保护作用
转化功能
杀伤能力
Astragalosides
Cadmium exposure
Enzyme activity
Immune function
T lymphocyte
NK cells
Protective effect
Transfor- mation ability
Killing activity
