摘要
目的：观察苓桂术甘汤与茵陈蒿汤合方（茵陈苓桂术甘汤）对非酒精性脂肪性肝炎大鼠细胞自噬的影响,初步探讨合方对非酒精性脂肪性肝炎大鼠的作用机制。方法：将SPF级SD大鼠随机分为正常对照组、模型组、茵陈苓桂术甘汤组、苓桂术甘汤组、茵陈蒿汤组,采用高脂饲料法建立NASH模型;高脂饲料饲养8周并同时给药,Western Blot法测定LC3II、LC3I表达量,Western Blot与RT-PCR法测定肝脏p62、ATG5蛋白与基因表达水平。结果：给药后,模型组大鼠肝脏ATG5表达量、LC3II/LC3I比值下降,p62表达量上升（P〈0.05）;与模型组比较,各给药组大鼠肝脏ATG5表达量、LC3II/LC3I比值有不同程度上升,p62表达量下降（P〈0.05,P〈0.01）。结论：茵陈苓桂术甘汤可能通过激活细胞自噬,从而达到改善氧化应激,防治NASH的目的。
Objective： To observe the effects of Yinchen Linggui Zhugan decoction on autophagy in rats with non-alcoholic steatohepatitis to investigate mechanism of the decoction. Methods： The SD rats were randomly divided into 5 groups： control group,model group and groups of Yinchen Linggui Zhugan Decoction,LGZGD and YCHD. Except for the control group,the rats of all the other groups received high-fat diet for making NASH models. And the treatment was given for 8 weeks. The comparative observation was carried out on the expressions of LC3 II/LC3 I,p62 and ATG5 via the methods of Western Blot or RT-PCR. Results： Compared with control group,the model group significantly decreased ATG5 and LC3 II/LC3 I,and increased p62（ P 〈0. 05）. The treatment groups showed higher expressive levels of ATG5 and LC3 II/LC3 I in the liver than the model group（ P 〈0. 01,P 〈0. 05）. The expression of p62 was lower in the treatment group. Conclusion： Yinchen Linggui Zhugan Decoction may improve oxidative stress to treat NASH via activating autophagy in the liver.
作者
郭一
李军祥
毛堂友
陈晨
谢添弘
韩亚飞
贾博宜
谭祥
王超
韩海啸
GUO Yi;LI Junxiang;MAO Tangyou;CHEN Chen;XIE Tianhong;HAN Yafei;JIA Boyi;TAN Xiang;WANG Chao;HAN Haixiao(Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第4期798-801,I0002,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81503549)
北京中医药大学自主选题中青年教师项目(2015-JYB-JSMS-110)
北京中医药大学自主选题在读研究生项目(2016-JYB-JSMS-XS170)
关键词
茵陈苓桂术甘汤
非酒精性脂肪性肝炎
细胞自噬
Yinchen Linggui Zhugan Decoction
non -alcoholic steatohepatitis
autophagy
