摘要
通过观察水稻叶色突变体ygr在不同生长时期叶色表型的动态变化,并对其进行温度敏感性试验,同时测定其光合特性和叶绿素荧光动力学参数。结果表明:在苗期和分蘖期时,突变体ygr的每一张新生叶黄化的程度明显,而到孕穗期时,其叶色与野生型的相似;4个不同恒温条件下的温度敏感性试验结果表明,突变体ygr是一个低温表达型叶色突变体;突变体ygr未转绿黄化叶的净光合速率(Pn)、光化学量子效率(Fv/Fm)、PSⅡ的激发能捕获效率(Fv'/Fm')、光下PSⅡ实际光化学量子效率(Φ_(PSⅡ))和电子传递速率(ETR)分别降低至8.87±1.67、0.70±0.04、0.27±0.07、0.17±0.05和89.84±28.29,均显著低于野生型。上述结果明确了突变体ygr的表型特征和光合特性,为突变体ygr的分子调控机制研究和促进其在水稻育种上的应用提供理论依据。
In this study, the dynamic changes of leaf color phenotype of a rice leaf mutant yellow-green revertible （ygr）at different growth stages were observed, and a temperature sensitive test was carried out, and its photosynthetic and chlorophyll fluorescence kinetic parameters were measured. Results showed that each new leaf of the mutant ygr showed yellower significantly both at seedling and tillering stages, while it had no difference at booting stage, compared with the wild type. Temperature sensitivity tests under four different constant temperature conditions showed that the leaf mutant phenotype of the mutant ygr was appeared at low temperature. Values of Pn, Fv/Fm, Fv/Fm＇, φPSⅡ and ETR in yellow leaves of the mutant ygr, which did not turn green, was 8.87±1.67, 0.70±0.04, 0.27±0.07, 0.17±0.05 and 89.84±28.29, respectively, and showed significantly lower than those of the wild type. These results clearly identified the phenotypie and photosynthetic characteristics of the mutant ygr. The research aimed to provide a theoretical basis for studying the molecular regulation mechanism of the mutant ygr and promoting its application in rice breeding.
作者
林秋云
沈建凯
谢振宇
贺治洲
尹明
袁佳
LIN Qiuyun 1, SHEN Jiankai1, XIE Zhenyu1, HE Zhlzhou1 , YIN Ming1, YUAN Jia2(1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China 2 College of Applied Science and technology of Hainan University, Danzhou, Hainan 571737, Chin)
出处
《热带作物学报》
CSCD
北大核心
2018年第2期267-273,共7页
Chinese Journal of Tropical Crops
基金
海南省财政科技计划资助(No.20163129)
中国热带农业科学院基本科研业务费专项资金(No.1630032016026)
中国热带农业科学院基本科研业务费专项资金(No.1630032016021)
关键词
水稻
叶色突变体
表型
光合速率
叶绿素荧光
rice
leaf mutant
phenotype
photosynthesis rate
chlorophyll fluorescence
