摘要
设计合成了一种基于罗丹明B为发光团的高选择性荧光探针。相对于其他常见金属离子,该探针对Cu2＋具有较好的选择性和灵敏度。优化实验条件,在乙醇-水溶液（V（乙醇）∶V（水）=4∶1,p H 7.3,50 mmol/L HEPES）测试介质中,探针对浓度在2.0×10^-6~7.0×10^-6mol/L范围内的Cu2＋呈现良好的线性响应,检出限为6.7×10^（-7）mol/L。Job＇s plot实验表明,探针与Cu2＋以1∶1结合。可逆实验表明,探针与Cu2＋的配合是可逆的,其识别原理为Cu2＋的加入导致探针P罗丹明内酯结构开环而发射出强的荧光。
A rhodamine derivative was synthesized and characterized as Cu2＋-selective fluorescent probe.Study showed that this proposed probe has good selectivity and sensitivity to Cu2＋compared to other tested metal ions.Probe displayed a good linear range of 2.0×10^（-6）~7.0×10^-6mol/L with a detection limit of 6.7×10^-7mol/L Cu2＋in ethanol-water solution（V（ethanol）∶V（water）=4∶1,p H 7.3,50 mmol/L HEPES）.The 1∶1 binding mode of probe with Cu2＋was proved by Job＇s plot experiment.The coordination of probe with Cu2＋is reversible,and the reaction mechanism is due to Cu2＋-induced ring opening of rhodamine spirolactam,which caused the fluorescence enhancement of probe P.
作者
李东洋
宋越超
袁梦婷
陈慧敏
林泽材
张军
LI Dong-yang;SONG Yue-chao;YUAN Meng-ting;CHEN Hui-min;LIN Ze-cai;ZHANG Jun(Laboratory of Environment Monitoring, School of Tropical and Laboratory Medicine;School of International Education, Hainan Medical University, Haikou 571199, China)
出处
《化学试剂》
CAS
北大核心
2018年第4期369-371,402,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(81560347,81660356,81760387)
大学生创新性课题项目(HYCX2015001,201611810059)
海南医学院重点扶持学科项目
