摘要
目的 山东省蚊虫标本中携带乙型脑炎病毒的情况.方法 2016年8-9月在山东省微山县、莒南县、垦利县采集蚊虫标本,用BHK-21细胞进行病毒分离,利用实时荧光PCR方法检测蚊虫标本中JEV的携带情况.对新分离的病毒进行生物学和生物信息学的鉴定.结果 在山东省共采集蚊虫标本8 418只,包括：三带喙库蚊、中华按蚊及骚扰阿蚊.分为81批研磨处理,共分离得到8株乙脑病毒,实时荧光RT-PCR特异性检测出23批乙脑病毒阳性蚊虫标本.基于E基因的系统进化分析表明新分离JEV均为基因Ⅰ型乙脑病毒,且与山东省以往分离的JEV同源性最高.结论 山东省蚊虫中以基因Ⅰ型乙脑病毒为主.
Objective To analysis the genotype of Japanese encephalitis virus （JEV） in mosquitoes from Shandong province.Methods Mosquitoes were collected between August and September in Weishan county,Junan county,and Kenli county of Shandong province in 2016.Viruses were isolated by BHK-21 cell and identified by molecular method.Real-Time RT-PCR was conducted to detect the Japanese encephalitis virus carried by the mosquitoes.Results A total of 8418 mosquitoes divided into 81 pools including 3 species,Culex tritaeniorhynchus,Anopheles sinensis and Armigeres obturbans.Eight Japanese encephalitis viruses were isolated;23 pools were positive by JEV specific real-time RT-PCR.Phylogenetic analysis on E sequence of JEV showed all JEV strains belonged to genotype Ⅰ JEV,and new strains that were homogenous with previous JEV strains isolated from Shandong.Conclusions Genotype Ⅰ JEV was the dominant genotype in Shandong province.
作者
张维嘉
林小娟
刘爱国
付士红
刘挂芳
邵楠
王倩莹
陶泽新
何英
雷雯雯
粱国栋
徐爱强
赵丽
王环宇
Zhang Weijia, Lin Xiaojuan, Liu Aiguo, Fu Shihong, Liu Guifang, Shao Nan, Wang Qianying, Tao Zexin, He Ying, Lei Wenwen, Liang Guodong, Xu Aiqiang, Zhao Li, Wang Huanyu(Department of Virology School of Public Health, Shandong University, Jinan 250012, China ;Dividsion of EPI Shandong Province Center for Disease Control and Prevention,Jinan 250012, China ;Dividsion of EPI Center for Disease Control and Prevention of Dongying City, Dongying 257091, China ;State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2018年第1期53-56,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家重点研发计划（2016YFD0500401）
传染病预防控制国家重点实验室重点项目（2015SKLID505）
山东预防医学会智飞疾病预防控制技术研究基金项目（LYH2017-05）
关键词
乙型脑炎病毒
进化分析
病毒分离
Japanese encephalitis virus
Phylogenetie analysis
Virus isolation
