摘要
目的：在中医＂肾脑相济＂理论的指导下,观察艾灸对围绝经期抑郁症大鼠海马Notch信号通路的影响作用。方法：选用SPF级雌性SD大鼠48个,随机分为空白组、模型组、西药组、艾灸组每组12只。采用去势合并慢性不可预见性刺激方法制造围绝经期抑郁症大鼠模型。治疗方法：空白组进行正常饲养,不予治疗。模型组不予治疗,固定并给予抓取刺激。西药组,给予盐酸氯米帕明片,连续28 d。艾灸组选取＂百会＂＂大椎＂和双侧＂肾俞＂进行艾灸治疗,连续28d。观察大鼠一般情况变化,动情周期,旷场实验,糖水偏好实验结果。运用ELISA法检测海马组织NICD表达。运用ELISA法及RT-PCR法检测海马组织中的Notch-1、Jagged 1、Hes 1,Mash 1及其mRNA表达。结果：造模后,除空白组外,模型组、西药组和艾灸组的一般情况差。与空白组相比,其余各组旷场总移动距离明显缩短,旷场中心区域停留时间明显减少,糖水偏好实验糖水消耗率明显下降,差异有统计学意义（P〈0.05）。经过28 d的艾灸和抗抑郁药治疗后,与模型组相比,西药组、艾灸组旷场总移动距离明显增大,旷场中心区域停留时间增加明显,糖水消耗率明显升高,差异有统计学意义（P〈0.05）。以ELISA方法检测大鼠海马Notch信号通路上相关蛋白NICD显示,经过28 d的治疗,与空白组相比较,模型组的NICD的表达明显低下,差异有统计学意义（P〈0.05）。经过艾灸治疗,与模型组比较,艾灸组的NICD表达恢复明显,差异有统计学意义（P〈0.05）。以ELISA和RT-PCR方法同时检测显示,经过28 d的治疗,与空白组相比较,模型组的Notch-1的表达明显低下,Jagged 1,Hes 1,Mash 1的表达明显上升,差异有统计学意义（P〈0.05）。经过艾灸和抗抑郁药治疗,与模型组比较,艾灸组和西药组的Notch-1表达恢复明显,艾灸组的Jagged 1和Hes1表达下降明显,艾灸组和西药组大鼠的Mash 1表达下降明显,差异有统计学意义（P〈0.05）。由于PCR在检测目的基因时从转录到翻译的过程中发生的错误,西药组大鼠的Jagged 1和Hes 1表达不见下降。结论：＂肾脑相济＂灸法,通过对围绝经期抑郁症大鼠海马Notch通路上有关蛋白的调控作用,促进海马细胞增生,对围绝经期抑郁症起到治疗作用。
Objective： To observe the effect of moxibustion on Notch signaling pathway in hippocampus of perimenopausal depression rats based on TCM brain-kidney axis theory. Methods： Forty-eight female SPF grade SD rats were randomly divided into control group,model group,western medicine group and moxibustion group. Each group had 12 rats. The perimenopausal depression rat model was made by ovariectomy combined with chronic unpredictable stimulation. The control group was fed normally without treatment. Model group was not treated,but fixed,and given grasping stimulation. The western medicine group was given Clomipramine Hydrochloride Tablets for 28 days. Moxibustion group was given moxibustion treatment for 28 days. The acupoints Baihui（ DU20）,Dazhui（ DU14） and bilateral Shenshu（ BL23） were selected. Results： After 21 days modeling,the general conditions of model,western medicine and moxibustion groups were poor. Compared with the control group,of all other groups,the total moving distance was dramatically shortened; the residence time in open field center area was significantly reduced; the sugar consumption was significantly decreased; the difference was statistically significant（ P〈0. 05）. After 28 days of moxibustion and antidepressant treatment,the general condition of the rats in western medicine group and moxibustion group were significantly improved. Compared with model group,western medicine and moxibustion group＇s total moving distance was dramatically increased;the residence time in open field center area was significantly increased; the sugar consumption was significantly increased and the difference was statistically significant（ P〈0. 05）. ELISA method was used to detect NICD. After 28 days of treatment,compared with the control group,the NICD expression of the model group was significantly low,and after treatment with moxibustion and antidepressants compared with model group,the NICD expression of moxibustion group and western medicine group was significantly increased and the difference was statistically significant（ P〈0. 05）. ELISA and Fluorescent quantitative PCR method were used to detect Notch-1,Jagged 1,Hes 1 and Mash 1. After 28 days of treatment,compared with the control group,the Notch-1 expression of the model group was significantly low; the Jagged 1,Hes 1 and Mash 1 were significantly high and the difference was statistically significant（ P〈0. 05）. After treatment with moxibustion and antidepressants treatment,compared with the model group,the Notch-1 expression of the moxibustion and western medicine group was significantly increased; the Jagged 1,Hes 1 and Mash 1 were significantly decreased and the difference was statistically significant（ P〈0. 05）. However,Jagged 1 and Hes 1 expression in western medicine group was not decreased due to the error occurred during the detection of the target gene from transcription to translation. Conclusion： Moxibustion on the acupoints Baihui（ DU20）,Dazhui（ DU14） and bilateral Shenshu（ BL23）could promote the rat hippocampal neurogenesis by regulating the rat hippocampal Notch signaling pathway.
作者
傅沈康
姜开妍
王玉
任路
FU Shenkang,JIANG Kaiyan, WANG Yu,REN Lu(Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, Chin)
出处
《辽宁中医杂志》
CAS
2018年第3期454-458,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81373718)
关键词
围绝经期抑郁症
艾灸
海马
NOTCH信号通路
perimenopausal depression
moxibustion
hippocampus
Notch signaling pathway
