摘要
绿僵菌是一类常见且极具应用价值的生物防治材料。目前绿僵菌分类系统取得了突破性的进展,然而新分类系统在国内尚未普遍采用。本文用该系统研究了近年来分离自大别山区僵虫的21株绿僵菌的物种多样性。在对大别山绿僵菌菌株的形态学研究基础上;对其转录延长因子(EF-1a)进行了进一步的PCR扩增、测序和同源性分析,并选用MEGA软件依据简约法构建EF-1a系统发育树。结果表明,21株菌株在新系统中分别属于5个种,即贵州绿僵菌、大孢绿僵菌、蚱蜢绿僵菌、黄绿绿僵菌瘿绵蚜变种和棕色绿僵菌,并对中国新纪录棕色绿僵菌进行了形态学的描述。该研究基本明确了大别山区的绿僵菌物种多样性,为生物防治菌株提供了科学鉴定方法和丰富种质资源。
Entomopathogenic fungi in the genus Metarhizium are very common and useful for biological control. The classification system of Metarhizium was made great progress so far. However, the new system has no systematic application in China. This paper systematically studied the species diversity of 21 Metarhizium strains isolated from insects in Dabie Mountains in Anhui Province in recent twenty years was investigated. Morphological characters of Metarhizium, like colonial morphology and the growth rate, conidiogenous structures, spore morphology and spore size were calculated. The transcription elongation factor（EF-1 a） was amplified and sequenced. We analysed the homology and constructed the phylogenetic tree of EF-1 a by Maximum Parsimony method with MEGA.The result showed that this 21 Metarhizium strains in new system were falling in five branches. They are belong to five species respectively, M. guizhouense, M. majus, M. acridum, M. flavoviride var. pemphigi, and a new record species, M. brunneum. And the morphological of new record species was described. The study clarified the Metarhizium species diversity in Dabie Mountains and provided a scientific method for identification and more strains for biocontrol.
作者
陈名君
章西
侯囡嵩
黄勃
CHEN Mingjun;ZHANG Xi;HOU Nansong;HUANG Bo(Anhui Province Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, Chin)
出处
《中国生物防治学报》
CSCD
北大核心
2018年第2期274-279,共6页
Chinese Journal of Biological Control
基金
安徽省高校自然科学研究项(KJ2016A844)
安徽农业大学学科骨干培养计划(2014XKPY-15)
