摘要
建立高效液相色谱分离测定500 g/L三环唑·咪鲜胺悬乳剂中有效成分含量的分析方法。采用ODS-C18反相色谱柱和二极管列阵检测器(检测波长225 nm),以甲醇-乙腈-水为流动相,对试样中的三环唑和咪鲜胺进行分离,外标法定量分析。三环唑和咪鲜胺线性相关系数分别为0.999 7和0.999 5,标准偏差分别为0.11和0.10,变异系数分别为0.56%和0.42%,平均回收率分别为99.51%和99.55%。该方法简便、快捷、有效地分离了样品中的三环唑和咪鲜胺,精密度和准确度高、线性关系好。
This paper describes a method to separate and carry out quantitative analysis of tricyclazole·prochloraz 500 g per L SE by HPLC.Tricyclazole·prochloraz was separated from the sample through ODS-C18 reversed phase column using a mixture of methanol acetonitrile and water as mobile phase,and detected by PDA detector at 225 nm.In addition,the results were quantified by external standard method. The linear correlation coefficients of tricyclazole · prochloraz were 0. 9997 and 0. 9995,the standard deviations were 0.11 and 0.10,the coefficients of variation were 0.56% and 0.42%,the average recoveries were 99.51% and 99.55%,respectively.This method is convenient,effective,has high precision and accuracy and good linearity.
作者
王广友
林波
吴培
李磊
WANG Guang-you;LIN Bo;WU Pei;LI Lei(Key Laboratory of Chemical Pesticide of Shandong Province,Shandong Academy of Pesticide Sciences,Jinan 250033,Chin)
出处
《齐鲁工业大学学报》
2018年第2期59-61,共3页
Journal of Qilu University of Technology
基金
山东省农业科学院农业科技创新工程(CXGC2016B12)
