摘要
目的探索外周血间充质干细胞联合多孔可吸收明胶海绵-自聚合肽纳米水凝胶复合支架对SD大鼠股骨髁骨缺损修复的效果,为骨缺损修复提供新策略。方法 30只8周龄的雌性SD大鼠随机分为3组,每组10只,A组为空白对照组,B组为多孔可吸收明胶海绵-自聚合肽纳米水凝胶复合支架组,C组为外周血间充质干细胞联合多孔可吸收明胶海绵-自聚合肽纳米水凝胶复合支架组。利用股骨髁骨缺损修复处石蜡切片苏木精-伊红染色、X线检查及Micro-CT扫描三维重建观察成骨效果。结果术后影像学检查示实验组具有较好的成骨修复效果。组织学检查发现,C组出现大量新生骨,而A、B两组仅可见少量散在新生骨。结论外周血间充质干细胞作为组织工程的种子细胞,联合多孔可吸收明胶海绵-自聚合肽纳米水凝胶复合支架具有较好的骨缺损修复能力,具有良好的应用前景,值得更加深入地研究。
Objective This study aimed to explore the effect of peripheral blood mesenchymal stem cellscombined with porous absorbable gelatin sponge/self assembling peptide composite scaffolds on SD rat femoral con-dyle bone defect reconstruction and provide a new strategy for the repair of bone defects. Methods 30 female SDrats,8 W age,were randomly divided into 3 groups,10 every group. The group A was blank control group,groupB was porous absorbable gelatin sponge/self assembling peptide composite scaffold group,and group C was periph-eral blood mesenchymal stem cells combined with porous absorbable gelatin sponge/self assembling peptide compos-ite scaffold group. The effect of osteogenesis was observed by paraffin section,hematoxylin eosin staining,X-rayexamination,and Micro-CT scanning in 3 dimensional reconstruction of femoral condyle defect. Results Imagingexamination showed that the experimental group had better osteogenesis effect. Histological examination showedthat a lot of new bone tissue was found in group C,while only a small amount of new bone was found in the groupof A and B. Conclusions The experiment shows that peripheral blood mesenchymal stem cells as the seed cellsfor tissue engineering,combined with porous absorbable gelatin sponge-self assembling peptide has better ability torepair bone defects,and has good application prospect,which is worthy of further research.
作者
张志明
刘椿
伍国锋
曹延林
金扬磊
王一涵
郭家松
朱立新
ZHANG Zhiming;LIU Chun;WU Guofeng;CAO Yanlin;JIN Yanglei;WANG Yihang;GUO Jiasong;ZHU Lixin(Department of Orthopedics, Zhu- jiang Hospital, Southern Medical University, Guangzhou 510280, China)
出处
《实用医学杂志》
CAS
北大核心
2018年第6期924-928,932,共6页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:816721401007225)
广东省自然科学基金项目(编号:2017A030313111
2014A030313339)
关键词
外周血间充质干细胞
骨缺损
可吸收明胶海绵
自聚肽水凝胶
组织工程
peripheral blood mesenchymal stem cells
bone defect
absorbable gelatin sponge
self assembling peptide
tissue engineering
