摘要
目的探讨脾酪氨酸激酶(spleen tyrosine kinase,SYK)基因启动子区-803A>T(rs290987)单核苷酸多态与汉族女性人群乳腺癌的相关性。方法采用病例-对照研究,以聚合酶链反应(PCR)联合DNA直接测序法检测57例乳腺癌患者和60例与之年龄相匹配的健康对照者syk基因启动子区-803位点的基因型及等位基因频率。采用ELISA方法定量检测受试者血清SYK水平。结果 -803A>T多态T等位基因频率在乳腺癌组明显高于对照组(χ~2=5.348,P=0.021)。与AA基因型相比(TA+TT)基因型可显著增加乳腺癌发病风险,OR值为2.87,95%CI=1.27~6.49。乳腺癌组血清SYK水平显著低于对照组(F=33.278,P<0.01)。结论北京地区汉族女性人群SYK基因-803位点基因型TA和TT是乳腺癌发病的危险因素。
Objective To evaluate the association of the SYK gene promoter -803A/T polymorphism and breast cancer sus- ceptibility during Han descent female population in Belling area. Methods In this case control study,genotype of SYK （A〉 T at -803,rs290987） was determined by polymerase chain reaction combined with DNA direct sequencing in blood samples of 57 breast cancel＂ cases and 60 age-matched healthy controls. They were all female. Genotype and allele frequency distribu- tions of 803A/T were determined and analyzed. ELISA test was performed to detect the serum level of SYK in the two groups. Results The frequencies of the T allele and TA＋TT genotypes of the -803A〉T were found to be significantly higher in the breast cancer patients in contrast to the healthy individuals of the control group（χ2 = 5. 348, P〈 0. 021）. Pa tients with the T allele （TA＋TT） had a 2.87-fold risk of developing breast cancer compared with those with the A allele （AA） （OR=2.87;95% CI= 1.27-6.49）. The test group had significantly decreased level of SYK in serum compared to normal controls （F=33. 278,P〈0.01）. Conclusion This study revealed that the genotype TA and TT of the SYK gene - 803 loci of Han female population in the Belling area are t＇he risk factors for the breast cancer.
作者
靳庆娥
苏建荣
JIN Qing-e;SU Jian-rong(Department of Clinical Laboratory, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China)
出处
《现代检验医学杂志》
CAS
2018年第2期5-7,共3页
Journal of Modern Laboratory Medicine
基金
首都医科大学基础-临床科研合作基金(编号:12JL35)
