摘要
目的观察抑制性寡核苷酸(TLE)对狼疮样综合征模型小鼠的治疗作用。方法采用空肠弯曲菌CJ-S131诱导构建红斑狼疮样综合征小鼠模型。将小鼠随机分为7组,即正常组、模型对照组、狼疮样模型组,抑制性寡核苷酸皮下给药(给药剂量分别为5、10、20、40μg)的4个组。检测TLE对小鼠脏器指数、血清抗自身抗体、血清肌酐和尿蛋白与尿肌酐比值,以及对肾损伤的影响。结果与模型组相比,TLE(给药剂量为20、40μg)能显著抑制血清抗双链DNA和抗核小体抗体的水平(P<0.05);TLE(给药剂量为10、20、40μg)明显改善肾病理和功能的损伤(P<0.05)。结论 TLE对小鼠狼疮样综合征有一定的保护作用。
AIM To determine the beneficial effects mental systemic lupus erythematosus-like syndrome in mice. into normal model control, lupus-like model and TLE treated immunized with CJ-S131 in Freund＇ s complete adjuvant. of inhibitory oligodeoxynucleotide （TLE） on experi- METHODS BALB/c mice were randomly divided （5, 10, 20 or40 μg/mouse ） groups and mice were Organ indexes were measured. Autoantibodies were detec- ted by enzyme-linked immunosorbent assays. Urine protein and creatinine were measured and kidneys were exam- ined by light microscopy. RESULTS Compared with lupus-like model group, treatment with TLE （20,40 μg/ mouse） significantly inhibited anti-dsDNA and anti-nucleosome antibody levels （P 〈 0.05 ）, and markedly amelio- rated the glomerular injury （ P 〈 0.05 ）. CONCLUSION These findings suggest that inhibitory oligodeoxynucle- otide has a beneficial effect on lupus-like syndrome induced by CJ-S131 in BALB/c mice.
作者
孙波峰
欧颖烨
陆叶
陈海霞
力弘
SUN Bofeng;OU Yingye;LU Ye;CHEN Haixia;LI Hong(Department of Pharmacology and Biochemiztry, School of Pharmacy, Fudan University, Shanghai 201203, China;Department of Internal Medicine, Wanggang Community Health Service, Pudong New District, Shanghai 201201, China)
出处
《中国临床药学杂志》
CAS
2018年第2期77-82,共6页
Chinese Journal of Clinical Pharmacy
关键词
抑制性寡核苷酸
狼疮
自身抗体
inhibitory oligodeoxynucleotide
lupus
autoantibody
