摘要
目的探讨藤茶提取物对人前列腺癌细胞LNCaP细胞增殖抑制的作用机制。方法 LNCaP细胞分为对照组、藤茶提取物不同浓度组,MTT法检测细胞的增殖抑制率,计算半数抑制浓度(IC50),流式细胞术Annexin V/PI双染法检测细胞凋亡率,Western-blot法检测PI3K/Akt通路PI3K、Akt、p-Akt、Bcl-2蛋白的表达。结果藤茶提取物在10~50μg/mL浓度范围内对LNCaP细胞增殖抑制作用随剂量的升高而升高,IC50为28.45μg/mL;与对照组比,藤茶提取物25、50μg/mL处理组LNCaP细胞凋亡率明显升高(P<0.05,P<0.01),p-Akt、Bcl-2蛋白表达显著下调(P<0.05,P<0.01)。结论藤茶提取物对LNCaP细胞具有增殖抑制作用,其机制可能是通过抑制PI3K/Akt细胞信号通路下调Bcl-2蛋白表达从而促进凋亡。
Objective To discuss the proliferation inhibition mechanism of LNCaP cells,the human prostate cancer cells,by Tengcha extract.Methods The LNCaP cells were treated with different dosages of Tengcha extract.The MTT colorimetric method was used to detect the inhibitory effects of cell viability.The apoptosis rate was tested by the Annexin V/PI fluorescent staining of flow cytometric method.The protein expression of PI3 K,AKT,p-AKT,and Bcl-2 protein were detected by Western blot analysis.Results Between 10μg/mL and 50μg/mL,Tengcha extract could significantly inhibit LNCaP cells,with dose-dependent manner.The IC50 was 28.45μg/mL.Compared with the control group,the apoptosis rate of LNCaP cells of 25μg/mL and 50μg/mL groups were significant higher.On the contrary,the protein expression of p-Akt and Bcl-2 of them were lower obviously.All above the results had dose-dependent manner.Conclusion Tengcha extract has proliferation inhibition and apoptosis inducement with LNCaP cells.This function may be concerned with the regulation of PI3 K/Akt signal pathway and lower Bcl-2 expression.
作者
姚欣
林静瑜
郭丹
周春权
YAO Xin;LIN Jingyu;GUO Dan;ZHOU Chunquan(Fujian Academy of Tradi tional Chinene Medicine, Fuzhou, Fujian 350003, Chin)
出处
《福建医药杂志》
CAS
2018年第2期105-107,共3页
Fujian Medical Journal
基金
福建省卫生教育联合攻关计划项目
WKJ-FJ-06
福建省卫计委中医药项目(WZZE201302)
