摘要
目的研究梓醇对甲状腺功能亢进症大鼠的治疗作用。方法通过皮下注射左旋甲状腺素钠(L-T4)诱导甲状腺功能亢进症模型,给药剂量为280 ng/g;将成模大鼠分为模型组,梓醇低(10μg/g)、中(20μg/g)、高(40μg/g)剂量组,甲巯咪唑组(10μg/g),丙硫氧嘧啶组(40μg/g)。于给药21 d后检测各组大鼠的心率、心重指数、脾脏指数;酶联免疫法检测三碘甲状腺原氨酸(T3)、甲状腺素(T4)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)和促甲状腺激素(TSH)、抗甲状腺微粒体抗体(TMAB)、甲状腺球蛋白抗体(TGAB)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)、白细胞介素2(IL-2)和白细胞介素10(IL-10)水平。结果与模型组比较,梓醇中剂量组和梓醇高剂量组大鼠体质量差、心率,T3、T4、FT3、FT4、TGAB,IL-2/IL-10,全心、左心、脾脏指数等指标均明显降低(P<0.05),而TSH明显升高(P<0.05)。结论梓醇具有一定的改善和治疗甲状腺功能亢进症的作用。
Objective To investigate the therapeutic effects of catalpol on hyperthyroidism in rats. Methods The hyperthyroid model was established by levothyroxine sodium（ L-T4） with subcutaneous injection at dose of 280 ng/g. After successful modeling,the model rats were divided into the model group,thiamazole group（ 10 μg/g）,propylthiouracil group（ 40 μg/g） and catalpol groups with low-（ 10 μg/g）,middle-（ 20 μg/g） and high-（ 40 μg/g） dose. After treatment for 21 days,the heart rate,heart weight index and spleen index of rats in each group were measured. The levels of triiodothyronine（ T3）,thyroxine（ T4）,free triiodothyronine（ FT3）,free thyroxine（ FT4）,thyroid stimulating hormone（ TSH）,thyroid microsomal antibody（ TMAB）,thyroglobulin antibody（ TGAB）,tumor necrosis factor-α（ TNF-α）,interleukin-6（ IL-6）,interleukin-2（ IL-2） and interleukin-10（ IL-10） were detected by enzyme-linked immunosorbent assay.Results Compared with the model group,the heart rate,the levels of T3,T4,FT3,FT4,TGAB,IL-2/IL-10,the index of whole heart,left heart and spleen were significantly decreased in the catalpol groups with middle-and high-dose（ P〈0.05）,while the TSH level was increased significantly（ P〈0.05）. Conclusion Catalpol shows certain improving and therapeutic effects on hyperthyroidism.
作者
张凡
雷瑜
邵一丹
郭飞龙
玄振玉
ZHANG Fan;LEI Yu;SHAO Yidan;GUO Feilong;XUAN Zhenyu(Shanghai University of Traditional Chinese Medicine ,Shanghai 201203, China)
出处
《上海中医药杂志》
2018年第4期81-85,共5页
Shanghai Journal of Traditional Chinese Medicine
