摘要
旨在探讨饲粮不同铜水平对雄性水貂铜代谢、血清微量元素含量、血脂和血清抗氧化指标的影响。本研究选取(60±3)日龄的健康雌性水貂80只,随机分为8个组,每组10个重复,分别饲喂在基础日粮中添加0(Control组)、4(Cu4组)、8(Cu8组)、16(Cu16组)、32(Cu32组)、64(Cu64组)、128(Cu128组)和256mg·kg-1(Cu256组)铜的试验饲粮。试验预试期7d,正试期45d。正试期开始第30天,每组选择8只水貂进行铜元素代谢指标测定;饲养试验结束后,采集血液样品进行血清生化指标测定。结果表明:1)水貂摄入铜、粪铜、尿铜和铜在体内的沉积量随日粮铜水平增加呈一次线性和二次曲线极显著增加(linear,quadratic,P<0.01)。2)水貂血清铜含量随日粮铜水平的增加而升高(linear,quadratic,P<0.01)。3)水貂血清总胆固醇(TC,linear,quadratic,P<0.01)和甘油三酯(TG,linear,P<0.05;quadratic,P<0.01)含量随日粮铜水平的增加呈线性和二次曲线降低。4)水貂血清铜蓝蛋白(CER)和铜锌超氧化物歧化酶(Cu-Zn SOD)活性随日粮铜水平的增加呈二次曲线先增加后降低(quadratic,P<0.01)。综合以上指标可见,水貂日粮添加铜可以增加体内铜的沉积量和血清铜含量,同时能够降低血清胆固醇和甘油三酯水平,提高抗氧化酶活性。
This experiment was conducted to study the effects of dietary copper levels on copper balance,serum trace element contents,serum lipid metabolism parameters and serum antioxidant indices in male mink.Eighty healthy（60±3）day-old male minks were randomly divided into 8 groups with 10 minks in each group.0（Control）,4（Cu4）,8（Cu8）,16（Cu16）,32（Cu32）,64（Cu64）,128（Cu128）and 256 mg·kg-1（Cu256）of copper levels were added in basic diet,respec-tively.The pre-test period lasted for 7 days,and the trial lasted for 45 days.On day 30 of the trail,eight animals from each treatment group were selected randomly to determine copper balance.Blood samples were collected from mink before sacrifice to measure the serum biochemical indices.The results showed that：1）Intake of copper,fecal copper,urinary copper,retention copper had a linear and quadratic（P〈0.01）increase with the increasing dietary copper level.2）There was a linear and quadratic（P〈0.01）effect of dietary copper level on plasma Cu concentrations.3）Serum TC（linear,quadratic,P〈0.01）,TG（linear,P〈0.05;quadratic,P〈0.01）contents were decreased with the increasing dietary copper level.4）There was a quadratic（P〈0.01）effect of dietary copper level on plasma ceruloplasmin concentration and Cu-Zn superoxide dismutase activity.These results indicate that supplementing Cu in the diet can increase Cu retention and plasma Cu concentrations,but reduce serum TC and TG levels,and improve antioxidant enzyme activity.
作者
吴学壮
杨颖
刘汇涛
高秀华
杨福合
戴四发
邢秀梅
WU Xue-zhuang;YANG Ying;LIU Hui-tao;GAO Xiu-hua;YANG Fu-he;DAI Si-fa;XING Xiu-mei(College of Animal Science, Anhui Science and Technology University, Bengbu 233100, China;Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第4期746-753,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然资源平台专项"特种经济动物种质资源共享平台"(2005DKA21102)
安徽省自然科学基金(1708085QC74)
安徽科技学院高层次人才引进项目(DKYJ201701)
农业部饲料生物技术重点实验室开放课题资助
安徽科技学院重点学科建设项目(AKZDXK2015B03)
关键词
水貂
铜
血清生化指标
脂类代谢
mink
copper
serum biochemical indices
lipid metabolism
