摘要
目的:借助网络药理学分析方法,研究速效救心丸主要活性成分对心肌缺血疾病的网络调控作用。方法:预测速效救心丸中生物利用度较好的化合物,整合Pharm Mapper和Mas等公共数据库提供的成分、靶点、通路信息,使用Cytoscape 3.1.0软件构建速效救心丸成分-靶点-通路的药理学网络。运用蛋白互作分析、子簇聚类算法和功能富集分析,来发现蛋白互作网络中的功能模块及其所参与的生物学过程。结果:速效救心丸的化学成分-靶点-通路网络图包括145个点和374条边。MCODE算法提取出得分≥3的5个子簇经功能富集分析(Bin Go)显示,这5个子簇参与的生物学过程主要有:脂质和固醇荷尔蒙的相关代谢、谷胱甘肽和氨基酸的相关代谢、炎症反应和免疫功能、心肌功能和能量供应及血压调节等方面。结论:本研究运用网络药理学的方法和技术,从分子网络层面阐释了速效救心丸主要活性成分通过调节心脏能量供应、脂质紊乱、免疫反应等方面发挥了多成分、多靶点、多途径相互协同治疗疾病的机制。
Objective To investigate the regulation effects of main active compounds in suxiaojiuxin pill on myocardial ischemia diseases,by the network pharmacology methodology.Method"Rules of five"and TPSA principles were applied to predict oral bioavailability of primary components of suxiaojiuxin pill.Information of components,targets and pathways from Pharm Mapper and Mas 3.0 platform were integrated to build the pharmacology network by Cytoscape 3.1.0,and protein-protein interaction,clustering algorithm（MCODE）and Bin Go analysis were applied to find critical targets,and important biology pathways.Results The components-targets-pathways network contains 145 nodes and 374 edges.MCODE algorithm extracted 5 clusters having at least 3 nodes and the further Bin Go analysis revealed that components of suxiaojiuxin pill mainly participate in response to lipid and steroid hormone,response to amino acid and glutathione,immune response and inflammatory response,energy supply and regulation of blood pressure.Conclusion This article reveals the multi-components,multi-targets and multi-pathways cooperate to improve myocardial ischemia diseases by suxiaojiuxin pill,from the view of molecular network
作者
雷伟
邓艳芳
姜民
白刚
Lei Wei;Deng Yanfang;Jiang Min;Bai Gang(College of Pharmacy Nankai University TianJin 30035)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第1期7-16,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金面上项目(81673637):川芎冰片药对"引经
行气
活血"协同增效分子机制研究
负责人:姜民
关键词
速效救心丸
网络药理学
蛋白互作分析
功能富集分析
Suxiao Jiuxin Pill, Network pharmacology, protein interaction analysis, Enrichment of functions
