摘要
目的探讨饲喂市售含铝油条对大鼠AD相关蛋白表达的影响及可能分子机制。方法将24只健康清洁级雄性SD大鼠按体重随机分为2组:饲喂含油条组和对照组(饲喂普通饲料),饲喂量都是15g/d,连续6个月。饲喂6个月后,采用酶联免疫法、分光光度计法,分析大鼠血清、海马组织中NOS、MDA、T-SOD、Aβ1-42、AchE、T-tau、P-tau及GSK-3β的含量或活性的变化。结果与对照组相比,6个月的饲喂含铝油条导致大鼠穿越原平台位置的次数减少(对照组与油条组分别为2.667±0.492和1.833±0.577)和在目的象限的活动时间缩短(对照组与油条组分别为50.089±3.423和43.521±2.140),血清中NOS活性升高(对照组与油条组分别为14.773±1.611和18.727±1.740)、T-SOD活性下降(对照组与油条组分别为275.959±6.405和250.659±6.918)及MDA含量升高(对照组与油条组分别为5.225±0.835和7.553±1.067),海马组织中T-SOD活性下降(对照组与油条组分别为138.894±4.697和107.121±10.641)及GSK-3β和Ach E活性升高(对照组分别为2.306±0.113和0.643±0.101,油条组分别为2.837±0.170和1.469±0.123)、Aβ1-42、T-tau及P-tau等含量则皆增加(对照组分别为88.417±1.803、106.224±4.260和98.271±9.269,油条组分别为99.621±2.056、133.551±4.766和115.125±8.105)(均P<0.01)。结论长期饲喂含铝油条会引起大鼠的记忆能力下降及以上AD相关组分的异常改变,从而引起AD发生的风险。
[Objective]To investigate both the effect and the possible molecular mechanism of commercially available fried bread stick containing aluminum（FCA）on expression of AD-associated proteins in rats.[Methods]24 healthy and clean male SD rats were randomly divided into two groups：FCA group and control group（feed ordinary feed）,the feeding amount was 15 g/d,and duration was six months.After 6 months of treatment,the contents and activities of NOS,MDA,T-SOD,Aβ1-42,Ach E,T-tau,P-tau and GSK-3β in serum and hippocampal tissue of rats were detected by the enzyme linked immunosorbent assay and spectrophotometer method.[Results]Compared with control group,the times through the original platform position of rats in FCA group reduced（2.667 ±0.492 in control group and 1.833 ±0.577 in FCA group）and the activity time in the target quadrant shortened（50.089 ±3.423 in control group and 43.521 ±2.140 in FCA group）.In serum,the activity of NOS increased in FCA group as compared with control group（14.773±1.611 in control group and 18.727 ±1.740 in FCA group）,the activity of T-SOD decreased（275.959±6.405 in control group and 250.659±6.918 in FCA group）,and the content of MDA increased（5.225±0.835 in control group and 7.553 ±1.067 in FCA group）.In hippocampal tissue,the activity of T-SOD reduced in FCA group as compared with control group（138.894±4.697 in control group and 107.121±10.641 in FCA group）,and the activities of GSK-3βand Ach E in FCA group（2.837±0.170 and 1.469±0.123）increased as compared with control group（2.306±0.113 and 0.643±0.101）,the contents of Aβ1-42,T-tau and P-tau in FCA group（99.621±2.056,133.551±4.766 and 115.125±8.105）increased as compared with control group（88.417 ±1.803,106.224 ±4.260 and98.271 ±9.269）（all P 〈0.01）.[Conclusion]The long-term feeding FCA can induce decline in memory ability and abnormal changes of AD related components mentioned above in rats,resulting in risk of AD.
作者
钱绘
李根亮
李韬
QIAN Hui;LI Gen-lian;LI Tao(Library;Biochemistry Department of Basic Medical Sciences School, Youjiang Medical College ,Baise Guangxi, 533000, China)
出处
《职业与健康》
CAS
2018年第5期604-607,共4页
Occupation and Health
基金
广西自然科学基金项目(2014GXNSFAA118255)
关键词
老年痴呆症
市售含铝油条
行为
血清
海马组织
Alzheimer’s disease
Commercially available fried bread stick containing aluminum
Behavior
Serum
Hippocampus tissue
